Large particle sorting to isolate live parasitic nematode eggs

Alfonso Schmidt, Tiffany Bouchery, Graham Le Gros, Kylie M. Price

Research output: Contribution to journalArticleOtherpeer-review

Abstract

Traditional jet-in-air cell sorters have been designed and optimized to isolate small particles such as mammalian lymphocytes with an average diameter of 10 µm. We discuss the practical considerations of setting up a conventional jetin-air cell sorter, using a 200-µm nozzle, to isolate the large parasitic nematode eggs of Nippostrongylus brasiliensis, with a maximum size of 60 µm. The eggs were separated based on light scattering properties, no fluorescent dye or molecule was required.

Original languageEnglish
Pages (from-to)11.21.1-11.21.15
Number of pages15
JournalCurrent Protocols in Cytometry
Volume2016
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Keywords

  • Cell sorting
  • Egg
  • Large particle
  • Live parasite
  • Nematode

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