Large particle sorting to isolate live parasitic nematode eggs

Alfonso Schmidt; Tiffany Bouchery; Graham Le Gros; Kylie M. Price

doi:10.1002/0471142956.cy1121s76

Large particle sorting to isolate live parasitic nematode eggs

Alfonso Schmidt, Tiffany Bouchery, Graham Le Gros, Kylie M. Price

Research output: Contribution to journal › Article › Other › peer-review

2 Citations (Scopus)

Abstract

Traditional jet-in-air cell sorters have been designed and optimized to isolate small particles such as mammalian lymphocytes with an average diameter of 10 µm. We discuss the practical considerations of setting up a conventional jetin-air cell sorter, using a 200-µm nozzle, to isolate the large parasitic nematode eggs of Nippostrongylus brasiliensis, with a maximum size of 60 µm. The eggs were separated based on light scattering properties, no fluorescent dye or molecule was required.

Original language	English
Pages (from-to)	11.21.1-11.21.15
Number of pages	15
Journal	Current Protocols in Cytometry
Volume	2016
DOIs	https://doi.org/10.1002/0471142956.cy1121s76
Publication status	Published - 1 Jan 2016
Externally published	Yes

Keywords

Cell sorting
Egg
Large particle
Live parasite
Nematode

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10.1002/0471142956.cy1121s76

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AB - Traditional jet-in-air cell sorters have been designed and optimized to isolate small particles such as mammalian lymphocytes with an average diameter of 10 µm. We discuss the practical considerations of setting up a conventional jetin-air cell sorter, using a 200-µm nozzle, to isolate the large parasitic nematode eggs of Nippostrongylus brasiliensis, with a maximum size of 60 µm. The eggs were separated based on light scattering properties, no fluorescent dye or molecule was required.

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KW - Nematode

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ER -

Large particle sorting to isolate live parasitic nematode eggs

Abstract

Keywords

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