Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers

Caroline X. Gao, Yuguo Li, Benjamin J Cowling, Daniel K W Chu, Heilbronn Cherie, Belinda Lloyd, J. S. Malik Peiris, Wendy Tsui, Alfred Kwong, Kitty Chan, Julian W. Tang

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearch


A standard shop display manikin was customised with a life-like inhalation cycle, to simulate a human recipient and exposed to a natural exhalation flows (breathing, talking, coughing) from influenza-infected volunteers. Any aerosolised droplets that were inhaled by the recipient manikin containing influenza were captured onto filters fitted into mouth of the manikin. These were tested for influenza RNA using a sensitive PCR (polymerase chain reaction) assay. No influenza RNA was detected in any other filter sample. This suggests that the viral load in aerosolized droplets produced by these natural human exhalation flows may be significantly less than that found in diagnostic swabs from the same source.

Original languageEnglish
Title of host publication13th International Conference on Indoor Air Quality and Climate
Subtitle of host publicationHong Kong, 7-12 July 2014
Place of PublicationSanta Cruz
PublisherInternational Society of Indoor Air Quality and Climate
Number of pages8
Publication statusPublished - 2014
EventInternational Conference on Indoor Air Quality and Climate (Indoor Air) 2014 - Grand Hall, Centenary Campus, The University of Hong Kong, Hong Kong
Duration: 7 Jul 201412 Jul 2014
Conference number: 13th


ConferenceInternational Conference on Indoor Air Quality and Climate (Indoor Air) 2014
Abbreviated titleIndoor Air 2014
Country/TerritoryHong Kong
Otherthe papers presented at this conference do not appear to have been peer reviewed. Have sent an email to some of the Monash authors requesting a copy of the reviewers comments
Internet address


  • Air-sampler
  • Droplet
  • Influenza
  • Inhalation
  • Transmission

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