Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers

Caroline X. Gao; Yuguo Li; Benjamin J Cowling; Daniel K W Chu; Heilbronn Cherie; Belinda Lloyd; J. S. Malik Peiris; Wendy Tsui; Alfred Kwong; Kitty Chan; Julian W. Tang

Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers

Caroline X. Gao, Yuguo Li, Benjamin J Cowling, Daniel K W Chu, Heilbronn Cherie, Belinda Lloyd, J. S. Malik Peiris, Wendy Tsui, Alfred Kwong, Kitty Chan, Julian W. Tang

Eastern Health Clinical School

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research

Abstract

A standard shop display manikin was customised with a life-like inhalation cycle, to simulate a human recipient and exposed to a natural exhalation flows (breathing, talking, coughing) from influenza-infected volunteers. Any aerosolised droplets that were inhaled by the recipient manikin containing influenza were captured onto filters fitted into mouth of the manikin. These were tested for influenza RNA using a sensitive PCR (polymerase chain reaction) assay. No influenza RNA was detected in any other filter sample. This suggests that the viral load in aerosolized droplets produced by these natural human exhalation flows may be significantly less than that found in diagnostic swabs from the same source.

Original language	English
Title of host publication	13th International Conference on Indoor Air Quality and Climate
Subtitle of host publication	Hong Kong, 7-12 July 2014
Place of Publication	Santa Cruz
Publisher	International Society of Indoor Air Quality and Climate
Pages	20-27
Number of pages	8
Publication status	Published - 2014
Event	International Conference on Indoor Air Quality and Climate (Indoor Air) 2014 - Grand Hall, Centenary Campus, The University of Hong Kong, Hong Kong Duration: 7 Jul 2014 → 12 Jul 2014 Conference number: 13th http://www.rehva.eu/events/event-detail/article/indoor-air-2014.html

Conference

Conference	International Conference on Indoor Air Quality and Climate (Indoor Air) 2014
Abbreviated title	Indoor Air 2014
Country/Territory	Hong Kong
Period	7/07/14 → 12/07/14
Other	the papers presented at this conference do not appear to have been peer reviewed. Have sent an email to some of the Monash authors requesting a copy of the reviewers comments
Internet address	http://www.rehva.eu/events/event-detail/article/indoor-air-2014.html

Keywords

Air-sampler
Droplet
Influenza
Inhalation
Transmission

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Gao, C. X., Li, Y., Cowling, B. J., Chu, D. K. W., Cherie, H., Lloyd, B., Peiris, J. S. M., Tsui, W., Kwong, A., Chan, K., & Tang, J. W. (2014). Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers. In 13th International Conference on Indoor Air Quality and Climate: Hong Kong, 7-12 July 2014 (pp. 20-27). International Society of Indoor Air Quality and Climate.

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title = "Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers",

abstract = "A standard shop display manikin was customised with a life-like inhalation cycle, to simulate a human recipient and exposed to a natural exhalation flows (breathing, talking, coughing) from influenza-infected volunteers. Any aerosolised droplets that were inhaled by the recipient manikin containing influenza were captured onto filters fitted into mouth of the manikin. These were tested for influenza RNA using a sensitive PCR (polymerase chain reaction) assay. No influenza RNA was detected in any other filter sample. This suggests that the viral load in aerosolized droplets produced by these natural human exhalation flows may be significantly less than that found in diagnostic swabs from the same source.",

keywords = "Air-sampler, Droplet, Influenza, Inhalation, Transmission",

author = "Gao, {Caroline X.} and Yuguo Li and Cowling, {Benjamin J} and Chu, {Daniel K W} and Heilbronn Cherie and Belinda Lloyd and Peiris, {J. S. Malik} and Wendy Tsui and Alfred Kwong and Kitty Chan and Tang, {Julian W.}",

year = "2014",

language = "English",

pages = "20--27",

booktitle = "13th International Conference on Indoor Air Quality and Climate",

publisher = "International Society of Indoor Air Quality and Climate",

note = "International Conference on Indoor Air Quality and Climate (Indoor Air) 2014, Indoor Air 2014 ; Conference date: 07-07-2014 Through 12-07-2014",

url = "http://www.rehva.eu/events/event-detail/article/indoor-air-2014.html",

}

Gao, CX, Li, Y, Cowling, BJ, Chu, DKW, Cherie, H, Lloyd, B, Peiris, JSM, Tsui, W, Kwong, A, Chan, K & Tang, JW 2014, Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers. in 13th International Conference on Indoor Air Quality and Climate: Hong Kong, 7-12 July 2014. International Society of Indoor Air Quality and Climate, Santa Cruz, pp. 20-27, International Conference on Indoor Air Quality and Climate (Indoor Air) 2014, Hong Kong, 7/07/14.

Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers. / Gao, Caroline X.; Li, Yuguo; Cowling, Benjamin J et al.
13th International Conference on Indoor Air Quality and Climate: Hong Kong, 7-12 July 2014. Santa Cruz: International Society of Indoor Air Quality and Climate, 2014. p. 20-27.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research

TY - GEN

T1 - Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers

AU - Gao, Caroline X.

AU - Li, Yuguo

AU - Cowling, Benjamin J

AU - Chu, Daniel K W

AU - Cherie, Heilbronn

AU - Lloyd, Belinda

AU - Peiris, J. S. Malik

AU - Tsui, Wendy

AU - Kwong, Alfred

AU - Chan, Kitty

AU - Tang, Julian W.

N1 - Conference code: 13th

PY - 2014

Y1 - 2014

N2 - A standard shop display manikin was customised with a life-like inhalation cycle, to simulate a human recipient and exposed to a natural exhalation flows (breathing, talking, coughing) from influenza-infected volunteers. Any aerosolised droplets that were inhaled by the recipient manikin containing influenza were captured onto filters fitted into mouth of the manikin. These were tested for influenza RNA using a sensitive PCR (polymerase chain reaction) assay. No influenza RNA was detected in any other filter sample. This suggests that the viral load in aerosolized droplets produced by these natural human exhalation flows may be significantly less than that found in diagnostic swabs from the same source.

AB - A standard shop display manikin was customised with a life-like inhalation cycle, to simulate a human recipient and exposed to a natural exhalation flows (breathing, talking, coughing) from influenza-infected volunteers. Any aerosolised droplets that were inhaled by the recipient manikin containing influenza were captured onto filters fitted into mouth of the manikin. These were tested for influenza RNA using a sensitive PCR (polymerase chain reaction) assay. No influenza RNA was detected in any other filter sample. This suggests that the viral load in aerosolized droplets produced by these natural human exhalation flows may be significantly less than that found in diagnostic swabs from the same source.

KW - Air-sampler

KW - Droplet

KW - Influenza

KW - Inhalation

KW - Transmission

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UR - https://www.isiaq.org/docs/paper/HP0702.pdf

M3 - Conference Paper

AN - SCOPUS:84924742066

SP - 20

EP - 27

BT - 13th International Conference on Indoor Air Quality and Climate

PB - International Society of Indoor Air Quality and Climate

CY - Santa Cruz

T2 - International Conference on Indoor Air Quality and Climate (Indoor Air) 2014

Y2 - 7 July 2014 through 12 July 2014

ER -

Lack of influenza transmission to an inhaling life-like manikin from naturally influenza-infected human volunteers

Abstract

Conference

Keywords

Access to Document

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