Laboratory Diagnosis of Dengue Infection: Current Techniques and Future Strategies

Dinesh Subedi, Andrew W. Taylor-Robinson

Research output: Contribution to journalReview ArticleOtherpeer-review


Dengue is an increasingly significant vector-borne infectious disease, with over 50 million cases reported in more than half the world’s recognised independent states. Dengue fever, dengue haemorrhagic fever and dengue shock syndrome are distinct clinical forms of an infection that is caused by Dengue Virus, a member of the Flaviviridae family. All four well characterized serotypes of the virus can cause the full spectrum of disease from asymptomatic infection to life-threatening symptoms. For effective prevention and/or treatment of disease symptoms, early and rapid detection of virus in specimens collected from clinically suspected persons is a requirement that remains challenging. A positive laboratory diagnosis is essential to confirm dengue virus infection and hence to inform patient therapy. Here, we consider the pros and cons of currently available methods for identification, ranging from conventional to sophisticated tests. Reports indicate the use of a variety of diagnostic methods of varying sensitivity, highlighting the necessity for standardisation and quality control. Several novel approaches are in development and demand further evaluation.
Original languageEnglish
Article number44045
Number of pages8
JournalOpen Journal of Clinical Diagnostics
Issue number1
Publication statusPublished - 2014
Externally publishedYes

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