Label-based and label-free strategies for protein quantitation

Sushma Anand, Monisha Samuel, Ching Seng Ang, Shivakumar Keerthikumar, Suresh Mathivanan

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

72 Citations (Scopus)

Abstract

The precise quantification of changes between various physiological states in a biological system is highly complex in nature. Over the past few years, in combination with classical methods, mass spectrometry based approaches have become an indispensable tool in deciphering exact abundance of proteins in composite mixtures. The technique is now well established and employs both label-based and label-free quantitation strategies. Label-based quantitation methods utilize stable isotope labels which are incorporated within the peptides, introducing an expectable mass difference within the two or more experimental conditions. In contrast, label-free proteomics quantitates both relative and absolute protein quantity by utilizing signal intensity and spectral counting of peptides. This chapter focuses on the commonly used quantitative mass spectrometry methods for high-throughput proteomic analysis.

Original languageEnglish
Title of host publicationProteome Bioinformatics
EditorsShivakumar Keerthikumar, Suresh Mathivanan
PublisherHumana Press
Chapter4
Pages31-43
Number of pages13
ISBN (Electronic)9781493967407
ISBN (Print)9781493967384
DOIs
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1549
ISSN (Print)1064-3745

Keywords

  • Label-free quantitation
  • Mass spectrometry
  • Quantitative proteomics
  • Stable isotope labeling

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