Lab on a chip sensor for rapid detection and antibiotic resistance determination of Staphylococcus aureus

Chathurika D. Abeyrathne, Duc H. Huynh, Thomas W. McIntire, Thanh C. Nguyen, Babak Nasr, Daniela Zantomio, Gursharan Chana, Iain Abbott, Peter Choong, Mike Catton, Efstratios Skafidas

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16 Citations (Scopus)

Abstract

The Gram-positive bacterium, Staphylococcus aureus (S. aureus), is a major pathogen responsible for a variety of infectious diseases ranging from cellulitis to more serious conditions such as septic arthritis and septicaemia. Timely treatment with appropriate antibiotic therapy is essential to ensure clinical defervescence and to prevent further complications such as infective endocarditis or organ impairment due to septic shock. To date, initial antibiotic choice is empirical, using a "best guess" of likely organism and sensitivity- an approach adopted due to the lack of rapid identification methods for bacteria. Current culture based methods take up to 5 days to identify the causative bacterial pathogen and its antibiotic sensitivity. This paper provides proof of concept for a biosensor, based on interdigitated electrodes, to detect the presence of S. aureus and ascertain its sensitivity to flucloxacillin rapidly (within 2 hours) in a cost effective manner. The proposed method is label-free and uses non-faradic measurements. This is the first study to successfully employ interdigitated electrodes for the rapid detection of antibiotic resistance. The method described has important potential outcomes of faster definitive antibiotic treatment and more rapid clinical response to treatment.

Original languageEnglish
Pages (from-to)1922-1929
Number of pages8
JournalAnalyst
Volume141
Issue number6
DOIs
Publication statusPublished - 21 Mar 2016
Externally publishedYes

Cite this

Abeyrathne, C. D., Huynh, D. H., McIntire, T. W., Nguyen, T. C., Nasr, B., Zantomio, D., Chana, G., Abbott, I., Choong, P., Catton, M., & Skafidas, E. (2016). Lab on a chip sensor for rapid detection and antibiotic resistance determination of Staphylococcus aureus. Analyst, 141(6), 1922-1929. https://doi.org/10.1039/c5an02301g