Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics

Anja Greule, Thierry Izoré, Dumitrita Iftime, Julien Tailhades, Melanie Schoppet, Yongwei Zhao, Madeleine Peschke, Iftekhar Ahmed, Andreas Kulik, Martina Adamek, Robert J.A. Goode, Ralf B. Schittenhelm, Joe A. Kaczmarski, Colin J. Jackson, Nadine Ziemert, Elizabeth H. Krenske, James J. De Voss, Evi Stegmann, Max J. Cryle

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Kistamicin is a divergent member of the glycopeptide antibiotics, a structurally complex class of important, clinically relevant antibiotics often used as the last resort against resistant bacteria. The extensively crosslinked structure of these antibiotics that is essential for their activity makes their chemical synthesis highly challenging and limits their production to bacterial fermentation. Kistamicin contains three crosslinks, including an unusual 15-membered A-O-B ring, despite the presence of only two Cytochrome P450 Oxy enzymes thought to catalyse formation of such crosslinks within the biosynthetic gene cluster. In this study, we characterise the kistamicin cyclisation pathway, showing that the two Oxy enzymes are responsible for these crosslinks within kistamicin and that they function through interactions with the X-domain, unique to glycopeptide antibiotic biosynthesis. We also show that the kistamicin OxyC enzyme is a promiscuous biocatalyst, able to install multiple crosslinks into peptides containing phenolic amino acids.

Original languageEnglish
Article number2613
Number of pages15
JournalNature Communications
Volume10
Issue number1
DOIs
Publication statusPublished - 13 Jun 2019

Keywords

  • biosynthesis
  • enzyme mechanisms
  • x-ray crystallography

Cite this

Greule, Anja ; Izoré, Thierry ; Iftime, Dumitrita ; Tailhades, Julien ; Schoppet, Melanie ; Zhao, Yongwei ; Peschke, Madeleine ; Ahmed, Iftekhar ; Kulik, Andreas ; Adamek, Martina ; Goode, Robert J.A. ; Schittenhelm, Ralf B. ; Kaczmarski, Joe A. ; Jackson, Colin J. ; Ziemert, Nadine ; Krenske, Elizabeth H. ; De Voss, James J. ; Stegmann, Evi ; Cryle, Max J. / Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics. In: Nature Communications. 2019 ; Vol. 10, No. 1.
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abstract = "Kistamicin is a divergent member of the glycopeptide antibiotics, a structurally complex class of important, clinically relevant antibiotics often used as the last resort against resistant bacteria. The extensively crosslinked structure of these antibiotics that is essential for their activity makes their chemical synthesis highly challenging and limits their production to bacterial fermentation. Kistamicin contains three crosslinks, including an unusual 15-membered A-O-B ring, despite the presence of only two Cytochrome P450 Oxy enzymes thought to catalyse formation of such crosslinks within the biosynthetic gene cluster. In this study, we characterise the kistamicin cyclisation pathway, showing that the two Oxy enzymes are responsible for these crosslinks within kistamicin and that they function through interactions with the X-domain, unique to glycopeptide antibiotic biosynthesis. We also show that the kistamicin OxyC enzyme is a promiscuous biocatalyst, able to install multiple crosslinks into peptides containing phenolic amino acids.",
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Greule, A, Izoré, T, Iftime, D, Tailhades, J, Schoppet, M, Zhao, Y, Peschke, M, Ahmed, I, Kulik, A, Adamek, M, Goode, RJA, Schittenhelm, RB, Kaczmarski, JA, Jackson, CJ, Ziemert, N, Krenske, EH, De Voss, JJ, Stegmann, E & Cryle, MJ 2019, 'Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics' Nature Communications, vol. 10, no. 1, 2613. https://doi.org/10.1038/s41467-019-10384-w

Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics. / Greule, Anja; Izoré, Thierry; Iftime, Dumitrita; Tailhades, Julien; Schoppet, Melanie; Zhao, Yongwei; Peschke, Madeleine; Ahmed, Iftekhar; Kulik, Andreas; Adamek, Martina; Goode, Robert J.A.; Schittenhelm, Ralf B.; Kaczmarski, Joe A.; Jackson, Colin J.; Ziemert, Nadine; Krenske, Elizabeth H.; De Voss, James J.; Stegmann, Evi; Cryle, Max J.

In: Nature Communications, Vol. 10, No. 1, 2613, 13.06.2019.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics

AU - Greule, Anja

AU - Izoré, Thierry

AU - Iftime, Dumitrita

AU - Tailhades, Julien

AU - Schoppet, Melanie

AU - Zhao, Yongwei

AU - Peschke, Madeleine

AU - Ahmed, Iftekhar

AU - Kulik, Andreas

AU - Adamek, Martina

AU - Goode, Robert J.A.

AU - Schittenhelm, Ralf B.

AU - Kaczmarski, Joe A.

AU - Jackson, Colin J.

AU - Ziemert, Nadine

AU - Krenske, Elizabeth H.

AU - De Voss, James J.

AU - Stegmann, Evi

AU - Cryle, Max J.

PY - 2019/6/13

Y1 - 2019/6/13

N2 - Kistamicin is a divergent member of the glycopeptide antibiotics, a structurally complex class of important, clinically relevant antibiotics often used as the last resort against resistant bacteria. The extensively crosslinked structure of these antibiotics that is essential for their activity makes their chemical synthesis highly challenging and limits their production to bacterial fermentation. Kistamicin contains three crosslinks, including an unusual 15-membered A-O-B ring, despite the presence of only two Cytochrome P450 Oxy enzymes thought to catalyse formation of such crosslinks within the biosynthetic gene cluster. In this study, we characterise the kistamicin cyclisation pathway, showing that the two Oxy enzymes are responsible for these crosslinks within kistamicin and that they function through interactions with the X-domain, unique to glycopeptide antibiotic biosynthesis. We also show that the kistamicin OxyC enzyme is a promiscuous biocatalyst, able to install multiple crosslinks into peptides containing phenolic amino acids.

AB - Kistamicin is a divergent member of the glycopeptide antibiotics, a structurally complex class of important, clinically relevant antibiotics often used as the last resort against resistant bacteria. The extensively crosslinked structure of these antibiotics that is essential for their activity makes their chemical synthesis highly challenging and limits their production to bacterial fermentation. Kistamicin contains three crosslinks, including an unusual 15-membered A-O-B ring, despite the presence of only two Cytochrome P450 Oxy enzymes thought to catalyse formation of such crosslinks within the biosynthetic gene cluster. In this study, we characterise the kistamicin cyclisation pathway, showing that the two Oxy enzymes are responsible for these crosslinks within kistamicin and that they function through interactions with the X-domain, unique to glycopeptide antibiotic biosynthesis. We also show that the kistamicin OxyC enzyme is a promiscuous biocatalyst, able to install multiple crosslinks into peptides containing phenolic amino acids.

KW - biosynthesis

KW - enzyme mechanisms

KW - x-ray crystallography

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DO - 10.1038/s41467-019-10384-w

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VL - 10

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