Kinetics of yeast detachment from controlled stainless steel surfaces

Magali Demilly, Yves Bréchet, Franz Bruckert, Laurence Boulangé

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16 Citations (Scopus)

Abstract

Using a radial flow chamber, we study Saccharomyces cerevisiae kinetics of detachment from stainless steel substrates. Samples of similar surface chemistry, but with different surface topologies are compared: mirror polished and electro-chemically etched. Different grain sizes (20, 40 and 100 μm) and different etching depths (100-650 nm) are tested. Cells are removed from the substrate according to a first-order kinetics defining two macroscopic parameters that depend on the applied stress: the detachment efficiency and the detachment rate constant. Whatever the surface topology, detachment occurs above a threshold and its rate is strongly stimulated by the applied stress. The detachment efficiency is characterized by the shear stress at which half of the cells detach and is independent of surface topology. In contrast, detachment is faster from etched than mirror polished surfaces. Finally, we also show the preferential adhesion of yeast cells to grains of <0 0 1> crystallographic orientation with respect to the surface.

Original languageEnglish
Pages (from-to)71-79
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume51
Issue number1
DOIs
Publication statusPublished - 1 Aug 2006
Externally publishedYes

Keywords

  • Adhesion
  • Saccharomyces cerevisiae
  • Stainless steel
  • Yeast

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