Kinetics of HIV-1 Replication and Intracellular Accumulation of Particles in HTLV-I-Transformed Cells

Rosemary Kiernan, John Marshall, Richard Bowers, Richard Doherty, Dale Mcphee

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Abstract

The replication kinetics of HIV were examined in HTLV-I-transformed MT-2 cells. The duration of the initial replication cycle was 20 hours, determined by the first detection of infectious progeny virus, development of syncytia, and production of viral RNA and protein. A phase of exponential virus production followed until 62 h postinfection. Cell death occurred in the final phase of infection during which infectious virus production remained constant even though viral RNA and protein production increased at an exponential rate. Accumulations of HIV particles were observed within cytoplasmic vacuoles of infected MT-2 cells. Although cell lysates contained high titers of infectious virus, our data show that an increasing proportion of particles produced late in infection were not infectious.

Original languageEnglish
Pages (from-to)743-752
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume6
Issue number6
DOIs
Publication statusPublished - 1 Jan 1990
Externally publishedYes

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