Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells

Santhosh V. Kumar, Pei X. Er, Kynan T. Lawlor, Ali Motazedian, Michelle Scurr, Irene Ghobrial, Alexander N. Combes, Luke Zappia, Alicia Oshlack, Edouard G. Stanley, Melissa H. Little

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36 Citations (Scopus)

Abstract

Kidney organoids have potential uses in disease modelling, drug screening and regenerative medicine. However, novel cost-effective techniques are needed to enable scaled-up production of kidney cell types in vitro. We describe here a modified suspension culture method for the generation of kidney micro-organoids from human pluripotent stem cells. Optimisation of differentiation conditions allowed the formation of micro-organoids, each containing six to ten nephrons that were surrounded by endothelial and stromal populations. Single cell transcriptional profiling confirmed the presence and transcriptional equivalence of all anticipated renal cell types consistent with a previous organoid culture method. This suspension culture micro-organoid methodology resulted in a three-to fourfold increase in final cell yield compared with static culture, thereby representing an economical approach to the production of kidney cells for various biological applications.

Original languageEnglish
Article numberdev172361
Number of pages11
JournalDevelopment
Volume146
Issue number5
DOIs
Publication statusPublished - Mar 2019
Externally publishedYes

Keywords

  • Kidney
  • Kidney micro-organoid
  • Nephron
  • Organoid
  • Pluripotent stem cell
  • Single cell profiling
  • Suspension culture

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