Kallikrein gene expression in the rat anterior pituitary

P. J. Fuller; J. A. Clements; P. L. Whitfeld; J. W. Funder

doi:10.1016/0303-7207(85)90125-X

Kallikrein gene expression in the rat anterior pituitary

P. J. Fuller, J. A. Clements, P. L. Whitfeld, J. W. Funder

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

The report of 'kallikrein-like' activity in the rat neuro-intermediate lobe (N-IL) and its possible involvement in pro-opiomelanocortin processing led us to explore the expression of the kallikrein gene(s) in the pituitary. Using ³²P-labelled rat pancreatic kallikrein cDNA, we have shown positive hybridization for rat anterior pituitary poly(A) ⁺ RNA, of identical size on Northern blots ( ~ 1.0 kb) to rat kidney poly(A) ⁺ RNA run in parallel. Prior adrenalectomy or ovariectomy decreased the level of kallikrein mRNA seen in the anterior pituitary; total RNA from rat N-IL showed no significant hybridization. On hybridization histochemistry the anterior pituitary was strongly positive, and the neural and intermediate lobes negative. The previously reported kallikrein-like activity in the N-IL is therefore probably due to a non-kallikrein kininogenase; in the anterior pituitary, kallikrein may have a physiological role in limited precursor proteolysis, but lack kininogen activity.

Original language	English
Pages (from-to)	99-105
Number of pages	7
Journal	Molecular and Cellular Endocrinology
Volume	39
Issue number	2
DOIs	https://doi.org/10.1016/0303-7207(85)90125-X
Publication status	Published - 1985

Keywords

anterior pituitary
hybridization histochemistry
kallikrein

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10.1016/0303-7207(85)90125-X

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title = "Kallikrein gene expression in the rat anterior pituitary",

abstract = "The report of 'kallikrein-like' activity in the rat neuro-intermediate lobe (N-IL) and its possible involvement in pro-opiomelanocortin processing led us to explore the expression of the kallikrein gene(s) in the pituitary. Using 32P-labelled rat pancreatic kallikrein cDNA, we have shown positive hybridization for rat anterior pituitary poly(A) + RNA, of identical size on Northern blots ( ~ 1.0 kb) to rat kidney poly(A) + RNA run in parallel. Prior adrenalectomy or ovariectomy decreased the level of kallikrein mRNA seen in the anterior pituitary; total RNA from rat N-IL showed no significant hybridization. On hybridization histochemistry the anterior pituitary was strongly positive, and the neural and intermediate lobes negative. The previously reported kallikrein-like activity in the N-IL is therefore probably due to a non-kallikrein kininogenase; in the anterior pituitary, kallikrein may have a physiological role in limited precursor proteolysis, but lack kininogen activity.",

keywords = "anterior pituitary, hybridization histochemistry, kallikrein",

author = "Fuller, {P. J.} and Clements, {J. A.} and Whitfeld, {P. L.} and Funder, {J. W.}",

year = "1985",

doi = "10.1016/0303-7207(85)90125-X",

language = "English",

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pages = "99--105",

journal = "Molecular and Cellular Endocrinology",

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T1 - Kallikrein gene expression in the rat anterior pituitary

AU - Fuller, P. J.

AU - Clements, J. A.

AU - Whitfeld, P. L.

AU - Funder, J. W.

PY - 1985

Y1 - 1985

N2 - The report of 'kallikrein-like' activity in the rat neuro-intermediate lobe (N-IL) and its possible involvement in pro-opiomelanocortin processing led us to explore the expression of the kallikrein gene(s) in the pituitary. Using 32P-labelled rat pancreatic kallikrein cDNA, we have shown positive hybridization for rat anterior pituitary poly(A) + RNA, of identical size on Northern blots ( ~ 1.0 kb) to rat kidney poly(A) + RNA run in parallel. Prior adrenalectomy or ovariectomy decreased the level of kallikrein mRNA seen in the anterior pituitary; total RNA from rat N-IL showed no significant hybridization. On hybridization histochemistry the anterior pituitary was strongly positive, and the neural and intermediate lobes negative. The previously reported kallikrein-like activity in the N-IL is therefore probably due to a non-kallikrein kininogenase; in the anterior pituitary, kallikrein may have a physiological role in limited precursor proteolysis, but lack kininogen activity.

AB - The report of 'kallikrein-like' activity in the rat neuro-intermediate lobe (N-IL) and its possible involvement in pro-opiomelanocortin processing led us to explore the expression of the kallikrein gene(s) in the pituitary. Using 32P-labelled rat pancreatic kallikrein cDNA, we have shown positive hybridization for rat anterior pituitary poly(A) + RNA, of identical size on Northern blots ( ~ 1.0 kb) to rat kidney poly(A) + RNA run in parallel. Prior adrenalectomy or ovariectomy decreased the level of kallikrein mRNA seen in the anterior pituitary; total RNA from rat N-IL showed no significant hybridization. On hybridization histochemistry the anterior pituitary was strongly positive, and the neural and intermediate lobes negative. The previously reported kallikrein-like activity in the N-IL is therefore probably due to a non-kallikrein kininogenase; in the anterior pituitary, kallikrein may have a physiological role in limited precursor proteolysis, but lack kininogen activity.

KW - anterior pituitary

KW - hybridization histochemistry

KW - kallikrein

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U2 - 10.1016/0303-7207(85)90125-X

DO - 10.1016/0303-7207(85)90125-X

M3 - Article

C2 - 3845024

AN - SCOPUS:0021927376

SN - 0303-7207

VL - 39

SP - 99

EP - 105

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 2

ER -

Kallikrein gene expression in the rat anterior pituitary

Abstract

Keywords

UN SDGs

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