TY - JOUR
T1 - Vibrio coralliirubri sp. nov., a new species isolated from mucus of red coral (Corallium rubrum) collected at Procida island, Italy
AU - Poli, Annarita
AU - Romano, Ida
AU - Mastascusa, Vincenza
AU - Buono, Lorena
AU - Orlando, Pierangelo
AU - Nicolaus, Barbara
AU - Leone, Luigi
AU - Hong, Kar Wai
AU - Chan, Kok Gan
AU - Goh, Kian Mau
AU - Pascual, Javier
N1 - Funding Information:
Acknowledgements The authors thank AMP ‘‘Regno di Nettuno’’ for authorization to sample the red coral colonies, Dr. Guido Villani for the sampling red coral at Punta Pizzaco (Procida island, Italy), the NMR Service of Institute of Biomolecular Chemistry of CNR (Pozzuoli, Italy) and Mrs. Maria Cristina Del Barone of IPCB-LaMEST laboratory of C.N.R. for SEM analysis. This work was supported by a grant from the Italian Ministry of Environment Territory and Sea (project title ‘‘Supporto tecnico gestionale in vista della Convenzione CITES sul Corallo Rosso mediterraneo e criteri di distinzione da altre specie di Corallo’’). Genome sequencing was supported by the University of Malaya via High Impact Research Grants (UM.C/625/1/HIR/MOHE/CHAN/01 [Grant No. A-000001-50001] and UM.C/625/1/HIR/MOHE/CHAN/ 14/1 [Grant No. H-50001-A000027]) awarded to Kok-Gan Chan. Kian Mau Goh is grateful for funding received from Universiti Teknologi Malaysia GUP (Grant 09H98).
Funding Information:
The authors thank AMP ?Regno di Nettuno? for authorization to sample the red coral colonies, Dr. Guido Villani for the sampling red coral at Punta Pizzaco (Procida island, Italy), the NMR Service of Institute of Biomolecular Chemistry of CNR (Pozzuoli, Italy) and Mrs. Maria Cristina Del Barone of IPCB-LaMEST laboratory of C.N.R. for SEM analysis. This work was supported by a grant from the Italian Ministry of Environment Territory and Sea (project title ?Supporto tecnico gestionale in vista della Convenzione CITES sul Corallo Rosso mediterraneo e criteri di distinzione da altre specie di Corallo?). Genome sequencing was supported by the University of Malaya via High Impact Research Grants (UM.C/625/1/HIR/MOHE/CHAN/01 [Grant No. A-000001-50001] and UM.C/625/1/HIR/MOHE/CHAN/14/1 [Grant No. H-50001-A000027]) awarded to Kok-Gan Chan. Kian Mau Goh is grateful for funding received from Universiti Teknologi Malaysia GUP (Grant 09H98). The authors declare no conflict of interest exists.
Publisher Copyright:
© 2018, Springer International Publishing AG, part of Springer Nature.
PY - 2018/7
Y1 - 2018/7
N2 - Strain Corallo1T was isolated from mucus of red coral (Corallium rubrum) at Punta Pizzaco (Procida island, Naples, Italy). It was characterised as a Gram-stain negative, motile, rod-shaped bacterium. Strain Corallo1T was found to show positive responses for cytochrome-c oxidase, catalase, reduction of nitrate and nitrite, β-galactosidase activity and hydrolysis of starch, xylan, peptone, Tween 40, Tween 80 and casein. Strain Corallo1T was found to be mesophilic, neutrophilic to alkalophilic and slightly halophilic. According to analysis of the almost-complete 16S rRNA gene, strain Corallo1T is closely related to Vibrio celticus (100% sequence similarity), Vibrio gigantis (100%), Vibrio crassostreae (99.7%), Vibrio artabrorum (99.7%) and Vibrio pomeroyi (99.6%). MLSA of five housekeeping genes (atpA, pyrH, recA, rpoA and rpoD) was performed to refine the phylogenetic relationships of strain Corallo1T. A draft genome sequence of strain Corallo1T was obtained. The DNA G+C content of this strain was determined to be 44.5 mol %. The major cellular fatty acids of strain Corallo1T are C16:1, n-C16:0 and C18:1, and the major isoprenoid ubiquinone is Q8. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain Corallo1T represents an independent genomospecies. Based on a polyphasic taxonomic characterisation, strain Corallo1T is concluded to represent a novel species of the genus Vibrio, for which the name Vibrio coralliirubri sp. nov. is proposed. The type strain is Corallo1T (= DSM 27495T = CIP 110630T).
AB - Strain Corallo1T was isolated from mucus of red coral (Corallium rubrum) at Punta Pizzaco (Procida island, Naples, Italy). It was characterised as a Gram-stain negative, motile, rod-shaped bacterium. Strain Corallo1T was found to show positive responses for cytochrome-c oxidase, catalase, reduction of nitrate and nitrite, β-galactosidase activity and hydrolysis of starch, xylan, peptone, Tween 40, Tween 80 and casein. Strain Corallo1T was found to be mesophilic, neutrophilic to alkalophilic and slightly halophilic. According to analysis of the almost-complete 16S rRNA gene, strain Corallo1T is closely related to Vibrio celticus (100% sequence similarity), Vibrio gigantis (100%), Vibrio crassostreae (99.7%), Vibrio artabrorum (99.7%) and Vibrio pomeroyi (99.6%). MLSA of five housekeeping genes (atpA, pyrH, recA, rpoA and rpoD) was performed to refine the phylogenetic relationships of strain Corallo1T. A draft genome sequence of strain Corallo1T was obtained. The DNA G+C content of this strain was determined to be 44.5 mol %. The major cellular fatty acids of strain Corallo1T are C16:1, n-C16:0 and C18:1, and the major isoprenoid ubiquinone is Q8. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain Corallo1T represents an independent genomospecies. Based on a polyphasic taxonomic characterisation, strain Corallo1T is concluded to represent a novel species of the genus Vibrio, for which the name Vibrio coralliirubri sp. nov. is proposed. The type strain is Corallo1T (= DSM 27495T = CIP 110630T).
KW - Polyphasic taxonomy
KW - Red coral
KW - Splendidus clade
KW - Vibrio
UR - http://www.scopus.com/inward/record.url?scp=85040021776&partnerID=8YFLogxK
U2 - 10.1007/s10482-017-1013-5
DO - 10.1007/s10482-017-1013-5
M3 - Article
C2 - 29299771
AN - SCOPUS:85040021776
SN - 0003-6072
VL - 111
SP - 1105
EP - 1115
JO - Antonie Van Leeuwenhoek
JF - Antonie Van Leeuwenhoek
IS - 7
ER -