Isolation, propagation, and differentiation of radial Glia-like neural progenitor cells in adherent cultures

This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the AKcyclo(RGDfC) adhesive peptide-conjugate that allows the application of fully defined culture conditions in which epidermal growth factor (EGF) represents the only growth factor supplement. RGL cells maintained that way can be efficiently differentiated into the three major CNS cell types: neurons, astrocytes, and oligodendrocytes.