Isolation, propagation, and differentiation of radial Glia-like neural progenitor cells in adherent cultures

Balazs Varga, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

Abstract

This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the AKcyclo(RGDfC) adhesive peptide-conjugate that allows the application of fully defined culture conditions in which epidermal growth factor (EGF) represents the only growth factor supplement. RGL cells maintained that way can be efficiently differentiated into the three major CNS cell types: neurons, astrocytes, and oligodendrocytes.

Original languageEnglish
Pages (from-to)706-712
Number of pages7
JournalCold Spring Harbor Protocols
Volume2017
Issue number9
DOIs
Publication statusPublished - 1 Sep 2017

Cite this

@article{3dc5bdb44b374fcaae9fc751cc42629e,
title = "Isolation, propagation, and differentiation of radial Glia-like neural progenitor cells in adherent cultures",
abstract = "This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the AKcyclo(RGDfC) adhesive peptide-conjugate that allows the application of fully defined culture conditions in which epidermal growth factor (EGF) represents the only growth factor supplement. RGL cells maintained that way can be efficiently differentiated into the three major CNS cell types: neurons, astrocytes, and oligodendrocytes.",
author = "Balazs Varga and Andras Nagy",
year = "2017",
month = "9",
day = "1",
doi = "10.1101/pdb.prot094177",
language = "English",
volume = "2017",
pages = "706--712",
journal = "Cold Spring Harbor Protocols",
issn = "1940-3402",
number = "9",

}

Isolation, propagation, and differentiation of radial Glia-like neural progenitor cells in adherent cultures. / Varga, Balazs; Nagy, Andras.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 9, 01.09.2017, p. 706-712.

Research output: Contribution to journalArticleOtherpeer-review

TY - JOUR

T1 - Isolation, propagation, and differentiation of radial Glia-like neural progenitor cells in adherent cultures

AU - Varga, Balazs

AU - Nagy, Andras

PY - 2017/9/1

Y1 - 2017/9/1

N2 - This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the AKcyclo(RGDfC) adhesive peptide-conjugate that allows the application of fully defined culture conditions in which epidermal growth factor (EGF) represents the only growth factor supplement. RGL cells maintained that way can be efficiently differentiated into the three major CNS cell types: neurons, astrocytes, and oligodendrocytes.

AB - This protocol describes the isolation of radial glia-like neural progenitors (RGLs) from embryonic and adult mouse brain. Their propagation as an adherent monolayer is greatly supported by use of the AKcyclo(RGDfC) adhesive peptide-conjugate that allows the application of fully defined culture conditions in which epidermal growth factor (EGF) represents the only growth factor supplement. RGL cells maintained that way can be efficiently differentiated into the three major CNS cell types: neurons, astrocytes, and oligodendrocytes.

UR - http://www.scopus.com/inward/record.url?scp=85028917281&partnerID=8YFLogxK

U2 - 10.1101/pdb.prot094177

DO - 10.1101/pdb.prot094177

M3 - Article

VL - 2017

SP - 706

EP - 712

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1940-3402

IS - 9

ER -