Isolation of stable aroA mutants of Salmonella typhi Ty2: Properties and preliminary characterisation in mice

Gordon Dougan, Duncan Maskell, Derek Pickard, Carlos Hormaeche

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Abstract

Derivatives of the Salmonella typhi strain Ty2 carrying stable mutations in the aroA gene were isolated. The mutations were generated by transducing an aroA::Tn 10 marker into Ty2 and selecting for derivatives which were tetracyline sensitive and dependent on aromatic compounds for growth. Isolates that did not revert to aromatic compound independence at a detectable frequency were obtained. An S. typhimurium derived aroA specific DNA probe was used to demonstrate the presence of DNA rearrangements in the aroA region of the chromosome of some of the S. typhi aroA mutants. Most of these isolates still expressed Vi antigen. Aromatic compound dependent mutants of S. typhi were less virulent in mice than S. typhi Ty2 following intraperitoneal challenge with bacteria suspended in mucin. Mice immunised with one of these mutants, named WBL85-1, were protected against a potentially lethal challenge of S. typhi Ty2.

Original languageEnglish
Pages (from-to)402-405
Number of pages4
JournalMolecular and General Genetics
Volume207
Issue number2-3
DOIs
Publication statusPublished - 1 May 1987
Externally publishedYes

Keywords

  • Aromatic
  • Salmonella
  • Typhoid
  • Vaccine

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