@inbook{9fbeb352beb3486d8a174ad727b80a9b,
title = "Isolation of reprogramming intermediates during generation of induced pluripotent stem cells from mouse embryonic fibroblasts",
abstract = "Mature cells of the body can be reprogrammed towards a pluripotent state by forced expression of the transcription factors Oct-4, Klf-4, Sox2, and C-Myc (OKSM) at very low efficiency. To study the reprogramming process in detail the rare intermediates of the reaction need to be separated from the bulk population. Using a genetically engineered reprogrammable mouse strain we describe how to isolate intermediates from reprogramming cultures of mouse embryonic fibroblasts via antibody labeling of cell surface markers and fluorescence-activated cell sorting (FACS).",
author = "Nefzger, {Christian M} and {Alaei Shehni}, Sara and Polo, {Jose M}",
year = "2015",
doi = "10.1007/978-1-4939-2848-4_17",
language = "English",
isbn = "9781493928477",
series = "Methods in Molecular Biology: Springer Protocols",
publisher = "Humana Press",
pages = "205 -- 218",
editor = "Verma, {Paul J} and Huseyin Sumer",
booktitle = "Cell Reprogramming",
address = "United States of America",
edition = "1st",
}