Isolation of mouse primary gastric epithelial cells to investigate the mechanisms of Helicobacter pylori associated disease

Le Son Tran, Richard L. Ferrero

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

4 Citations (Scopus)

Abstract

The gastrointestinal epithelium provides the first line of defense against invading pathogens, among which Helicobacter pylori is linked to numerous gastric pathologies, including chronic gastritis and cancer. Primary gastric epithelial cells represent a useful model for the investigation of the underlying molecular and cellular mechanisms involved in these H. pylori associated diseases. In this chapter, we describe a method for the isolation of primary gastric epithelial cells from mice and detection of epithelial cell adhesion molecule (EpCAM) expression in the isolated cells.

Original languageEnglish
Title of host publicationInflammation and Cancer
PublisherHumana Press
Pages119-126
Number of pages8
DOIs
Publication statusPublished - 11 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1725
ISSN (Print)1064-3745

Keywords

  • EpCAM
  • Gastric epithelial cells
  • Helicobacter pylori
  • Primary culture

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