Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis

Nigora Mukhamedova; Kevin Huynh; Hann Low; Peter J. Meikle; Dmitri Sviridov

doi:10.21769/BioProtoc.3670

Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis

Nigora Mukhamedova, Kevin Huynh, Hann Low, Peter J. Meikle, Dmitri Sviridov

Research output: Contribution to journal › Article › Other › peer-review

5 Citations (Scopus)

Abstract

Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.

Original language	English
Article number	e3670
Number of pages	19
Journal	bio-protocol
Volume	10
Issue number	13
DOIs	https://doi.org/10.21769/BioProtoc.3670
Publication status	Published - 5 Jul 2020
Externally published	Yes

Keywords

Cholesterol
Lipid rafts
Lipidomics
Lipids
Membrane

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10.21769/BioProtoc.3670

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title = "Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis",

abstract = "Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.",

keywords = "Cholesterol, Lipid rafts, Lipidomics, Lipids, Membrane",

author = "Nigora Mukhamedova and Kevin Huynh and Hann Low and Meikle, \{Peter J.\} and Dmitri Sviridov",

note = "Funding Information: The authors were supported by the grant from the National Institutes of Health (HL131473 to DS). Publisher Copyright: Copyright {\textcopyright} 2020 The Authors; exclusive licensee Bio-protocol LLC.",

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doi = "10.21769/BioProtoc.3670",

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TY - JOUR

T1 - Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis

AU - Mukhamedova, Nigora

AU - Huynh, Kevin

AU - Low, Hann

AU - Meikle, Peter J.

AU - Sviridov, Dmitri

N1 - Funding Information: The authors were supported by the grant from the National Institutes of Health (HL131473 to DS). Publisher Copyright: Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.

PY - 2020/7/5

Y1 - 2020/7/5

N2 - Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.

AB - Lipid rafts are distinct liquid-ordered domains of plasma membranes of most eukaryotic cells providing platform for signaling pathways. Lipid composition of rafts is critical for their structural integrity and for regulation of signaling pathways originating from rafts. Here we provide a protocol to isolate lipid rafts from cultured human and animal cells and comprehensively analyse their lipid composition.

KW - Cholesterol

KW - Lipid rafts

KW - Lipidomics

KW - Lipids

KW - Membrane

UR - https://www.scopus.com/pages/publications/85111129431

U2 - 10.21769/BioProtoc.3670

DO - 10.21769/BioProtoc.3670

M3 - Article

C2 - 33659340

AN - SCOPUS:85111129431

SN - 2331-8325

VL - 10

JO - bio-protocol

JF - bio-protocol

IS - 13

M1 - e3670

ER -

Isolation of Lipid Rafts from Cultured Mammalian Cells and Their Lipidomics Analysis

Abstract

Keywords

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