Isolation of Jannaschia sedimins sp. nov. from East Coast of China: Bacterial Taxonomy and Antimicrobial Resistance Analysis

Sha Sha, Shuqian Li, Lihua Zhao, Jinxin Zhao, Guanjun Chen

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A Gram-stain-negative, facultatively aerobic, pink and oval bacterium, designed OS4T, was isolated from a sediment sample taken from a coastal zone in China. The growth of OS4T occurred at 20–37C (optimal 25C), pH 7.0–8.5 (optimal pH 8.0), in 0–5.0% (w/v) NaCl (optimal 2.0%). According to the phylogenetic analysis, strain OS4T showed the highest sequence similarity (96.04%) with Jannaschia aquimarina GSW-M26T and shared 94.98% similarity with the type species of genus Jannaschia-strain J. helgolandensis 14858T. Chemotaxonomic analysis showed that the sole respiratory quinone was ubiquinone 10, and the major fatty acids (>5.0%) included C18:1 ω6c/ω7c, C18:0, and C10:0 3OH. The polar lipids consist of three phospholipids, two unknown amino-lipids, and four unknown glycerolipids. The DNA G + C content was 72.7 mol%. Based on the evidence presented in this study, strain OS4T represents a novel species of the genus Jannaschia, for which the name Jannaschia sedimins sp. nov. is proposed. The type strain is OS4T (=KCTC 82508T = MCCC 1K03755T). Both the phenotypic and the genetic analysis on the antimicrobial resistance genes indicate that OS4T is resistant to a wide range of classes of antibiotics, which highlights that the ocean could potentially serve as the natural reservoir of antimicrobial resistance genes.

Original languageEnglish
Article number2883
Number of pages9
JournalApplied Sciences
Volume12
Issue number6
DOIs
Publication statusPublished - Mar 2022

Keywords

  • 16S rRNA 4
  • Antimicrobial resistance 1
  • Jannaschia
  • Microbial communities 2
  • Polyphasic taxonomy 3

Cite this