Abstract
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology - Human Retrovirus Protocols: Virology and Molecular Biology |
| Editors | Tuofu Zhu |
| Place of Publication | USA |
| Publisher | Humana Press |
| Pages | 25 - 33 |
| Number of pages | 9 |
| Edition | 304 |
| ISBN (Print) | 9781588294951 |
| DOIs | |
| Publication status | Published - 2005 |
Cite this
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Isolation of human immunodeficiency virus Type 1 from peripheral blood monocytes. / Gorry, Paul R; Sonza, Secondo; Kedzierska, Katherine; Crowe, Suzanne Mary.
Methods in Molecular Biology - Human Retrovirus Protocols: Virology and Molecular Biology. ed. / Tuofu Zhu. 304. ed. USA : Humana Press, 2005. p. 25 - 33.Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other
TY - CHAP
T1 - Isolation of human immunodeficiency virus Type 1 from peripheral blood monocytes
AU - Gorry, Paul R
AU - Sonza, Secondo
AU - Kedzierska, Katherine
AU - Crowe, Suzanne Mary
PY - 2005
Y1 - 2005
N2 - Human immunodeficiency virus type 1 (HIV-1) can infect circulating peripheral blood monocytes and resting CD4+ T lymphocytes despite sustained suppression of plasma viremia to undetectable levels. These persistently infected cell populations pose a barrier for virus eradication by highly active antiretroviral therapy (HAART), and are a significant reservoir of HIV-1 contributing to viral rebound following cessation or failure of HAART. This chapter provides a protocol for isolating replication-competent HIV-1 from peripheral blood monocytes of HIV-1-infected individuals, including those with sustained plasma HIV-1 RNA levels below 50 copies/mL, by co-culture with CD8-depleted, phytohemagglutinin-activated donor peripheral blood mononuclear cells. In our laboratory, this protocol has the sensitivity to achieve a success rate of positive HIV-1 isolation in approx 70 of cases. The study of HIV-1 strains harbored by peripheral blood monocytes of patients undergoing HAART will contribute to the understanding of viral persistence in cellular reservoirs that impede effective HAART.
AB - Human immunodeficiency virus type 1 (HIV-1) can infect circulating peripheral blood monocytes and resting CD4+ T lymphocytes despite sustained suppression of plasma viremia to undetectable levels. These persistently infected cell populations pose a barrier for virus eradication by highly active antiretroviral therapy (HAART), and are a significant reservoir of HIV-1 contributing to viral rebound following cessation or failure of HAART. This chapter provides a protocol for isolating replication-competent HIV-1 from peripheral blood monocytes of HIV-1-infected individuals, including those with sustained plasma HIV-1 RNA levels below 50 copies/mL, by co-culture with CD8-depleted, phytohemagglutinin-activated donor peripheral blood mononuclear cells. In our laboratory, this protocol has the sensitivity to achieve a success rate of positive HIV-1 isolation in approx 70 of cases. The study of HIV-1 strains harbored by peripheral blood monocytes of patients undergoing HAART will contribute to the understanding of viral persistence in cellular reservoirs that impede effective HAART.
UR - http://www.springerlink.com/content/m172v73932014043/fulltext.pdf
U2 - 10.1385/1-59259-907-9:025
DO - 10.1385/1-59259-907-9:025
M3 - Chapter (Book)
SN - 9781588294951
SP - 25
EP - 33
BT - Methods in Molecular Biology - Human Retrovirus Protocols: Virology and Molecular Biology
A2 - Zhu, Tuofu
PB - Humana Press
CY - USA
ER -