Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage

T. Y. Thung; B. M.F. Siti Norshafawatie; J. M.K.J.K. Premarathne; W. S. Chang; Y. Y. Loo; C. H. Kuan; C. Y. New; A. Ubong; O. S.B. Ramzi; N. A. Mahyudin; F. B. Dayang; W. M.R. Che Wan Jasimah; R. Son

Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage

T. Y. Thung, B. M.F. Siti Norshafawatie, J. M.K.J.K. Premarathne, W. S. Chang, Y. Y. Loo, C. H. Kuan, C. Y. New, A. Ubong, O. S.B. Ramzi, N. A. Mahyudin, F. B. Dayang, W. M.R. Che Wan Jasimah, R. Son

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Abstract

Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 10⁸ to 10¹¹ PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods.

Original language	English
Pages (from-to)	450-454
Number of pages	5
Journal	International Food Research Journal
Volume	24
Issue number	1
Publication status	Published - Feb 2017
Externally published	Yes

Keywords

Agar overlay assay
Bacteriophage
Food-borne pathogen
Isolation

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Thung, T. Y., Siti Norshafawatie, B. M. F., Premarathne, J. M. K. J. K., Chang, W. S., Loo, Y. Y., Kuan, C. H., New, C. Y., Ubong, A., Ramzi, O. S. B., Mahyudin, N. A., Dayang, F. B., Che Wan Jasimah, W. M. R., & Son, R. (2017). Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage. International Food Research Journal, 24(1), 450-454.

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abstract = "Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods.",

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AU - Thung, T. Y.

AU - Siti Norshafawatie, B. M.F.

AU - Premarathne, J. M.K.J.K.

AU - Chang, W. S.

AU - Loo, Y. Y.

AU - Kuan, C. H.

AU - New, C. Y.

AU - Ubong, A.

AU - Ramzi, O. S.B.

AU - Mahyudin, N. A.

AU - Dayang, F. B.

AU - Che Wan Jasimah, W. M.R.

AU - Son, R.

PY - 2017/2

Y1 - 2017/2

N2 - Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods.

AB - Bacteriophages are the viruses of bacteria and are widely distributed in the biosphere, exhibiting dramatic manifestations both in liquid cultures and on solid media. In this study, bacteriophages were isolated from different types of food (beef, chicken meats, cucumber, lettuce, clam, cockles and shrimp) and sewage samples using 6 reference pathogen strains (Salmonella Enteritidis, Salmonella Typhimurium, Campylobacter jejuni, Vibrio parahaemolyticus, Listeria monocytogenes and Escherichia coli). A total of 29 bacteriophage isolates were obtained and further examined for titer via agar overlay assay. The titers were determined within the range of 108 to 1011 PFU/mL. Our results showed that diverse of bacteriophages are naturally present in a variety of foods.

KW - Agar overlay assay

KW - Bacteriophage

KW - Food-borne pathogen

KW - Isolation

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SN - 1985-4668

VL - 24

SP - 450

EP - 454

JO - International Food Research Journal

JF - International Food Research Journal

IS - 1

ER -

Isolation of food-borne pathogen bacteriophages from retail food and environmental sewage

Abstract

Keywords

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