Isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp

Marie Christine Ralet, Jean François Thibault, Craig B. Faulds, Gary Williamson

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136 Citations (Scopus)

Abstract

Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2 chromatography in aqueous solvents. Feruloylated arabinose di-, tri-, hexa-, hepta-, and octa-saccharides as well as feruloylated galactose disaccharides were obtained after hydrolysis of the pulp with a mixture of fungal carbohydrases (Driselase). Feruloylated arabinose and galactose monosaccharides were obtained through mild acid hydrolyses. Both arabinose and galactose residues in the side-chains are feruloylated, 50-55% of the feruloyl groups being linked to arabinose residues and 45-50% to galactose residues. It is concluded that 1 out of 56 arabinose residues and 1 out of 16 galactose residues present as pectic side-chains in sugar-beet pulp carry a feruloyl group.

Original languageEnglish
Pages (from-to)227-241
Number of pages15
JournalCarbohydrate Research
Volume263
Issue number2
DOIs
Publication statusPublished - 17 Oct 1994
Externally publishedYes

Keywords

  • Arabinans
  • Beet pulp
  • Ferulic acid
  • Galactans
  • Pectins

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