TY - JOUR
T1 - Isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp
AU - Ralet, Marie Christine
AU - Thibault, Jean François
AU - Faulds, Craig B.
AU - Williamson, Gary
PY - 1994/10/17
Y1 - 1994/10/17
N2 - Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2 chromatography in aqueous solvents. Feruloylated arabinose di-, tri-, hexa-, hepta-, and octa-saccharides as well as feruloylated galactose disaccharides were obtained after hydrolysis of the pulp with a mixture of fungal carbohydrases (Driselase). Feruloylated arabinose and galactose monosaccharides were obtained through mild acid hydrolyses. Both arabinose and galactose residues in the side-chains are feruloylated, 50-55% of the feruloyl groups being linked to arabinose residues and 45-50% to galactose residues. It is concluded that 1 out of 56 arabinose residues and 1 out of 16 galactose residues present as pectic side-chains in sugar-beet pulp carry a feruloyl group.
AB - Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by Sephadex LH-20 and Biogel P-2 chromatography in aqueous solvents. Feruloylated arabinose di-, tri-, hexa-, hepta-, and octa-saccharides as well as feruloylated galactose disaccharides were obtained after hydrolysis of the pulp with a mixture of fungal carbohydrases (Driselase). Feruloylated arabinose and galactose monosaccharides were obtained through mild acid hydrolyses. Both arabinose and galactose residues in the side-chains are feruloylated, 50-55% of the feruloyl groups being linked to arabinose residues and 45-50% to galactose residues. It is concluded that 1 out of 56 arabinose residues and 1 out of 16 galactose residues present as pectic side-chains in sugar-beet pulp carry a feruloyl group.
KW - Arabinans
KW - Beet pulp
KW - Ferulic acid
KW - Galactans
KW - Pectins
UR - http://www.scopus.com/inward/record.url?scp=0028518976&partnerID=8YFLogxK
U2 - 10.1016/0008-6215(94)00175-8
DO - 10.1016/0008-6215(94)00175-8
M3 - Article
C2 - 7805051
AN - SCOPUS:0028518976
SN - 0008-6215
VL - 263
SP - 227
EP - 241
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 2
ER -