Isolation and culture of adult intestinal, gastric, and liver organoids for cre-recombinase-mediated gene deletion

Dustin J. Flanagan, Renate H.M. Schwab, Bang M. Tran, Toby J. Phesse, Elizabeth Vincan

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Abstract

The discovery of Lgr5 as a marker of adult stem cells meant that stem cell populations could be purified and studied in isolation. Importantly, when cultured under the appropriate conditions these stem cells form organoids in tissue culture that retain many features of the tissue of origin. The organoid cultures are accessible to genetic and biochemical manipulation, bridging the gap between in vivo mouse models and conventional tissue culture. Here we describe robust protocols to establish organoids from gastrointestinal tissues (stomach, intestine, liver) and Cre-recombinase mediated gene manipulation in vitro.

Original languageEnglish
Title of host publicationOrganoids
Subtitle of host publicationStem Cells, Structure, and Function
EditorsKursad Turksen
Place of PublicationNew York, NY
PublisherHumana Press
Pages123-133
Number of pages11
ISBN (Electronic)9781493976171
ISBN (Print)9781493976164
DOIs
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1576
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • 3D organoid culture
  • Cre-recombinase
  • Gastric organoids
  • Intestinal organoids
  • Lgr5
  • Liver organoids

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