Isolation and Cultivation of Diplonemids

Daria Tashyreva; Galina Prokopchuk; Orsola Iorillo; Matus Valach; Ingrid Škodová-Sveráková; Dong Woo Shin; James A. Letts; Gertraud Burger; Julius Lukeš; Drahomíra Faktorová

doi:10.1007/978-1-0716-5142-1_2

Isolation and Cultivation of Diplonemids

Daria Tashyreva
, Galina Prokopchuk
, Orsola Iorillo
, Matus Valach
, Ingrid Škodová-Sveráková
, Dong Woo Shin
, James A. Letts
, Gertraud Burger
, Julius Lukeš
, Drahomíra Faktorová

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.

Original language	English
Title of host publication	Euglenozoa
Subtitle of host publication	Methods and Protocols
Editors	Paul A.M. Michels, Michael L. Ginger, Anna Karnkowska, Laura-Isobel McCall, Ariel M. Silber
Place of Publication	New York NY USA
Publisher	Humana Press
Chapter	2
Pages	25-40
Number of pages	16
Volume	1
Edition	1st
ISBN (Electronic)	9781071651421
ISBN (Print)	9781071651414, 9781071651445
DOIs	https://doi.org/10.1007/978-1-0716-5142-1_2
Publication status	Published - 2026
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	3013
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Axenization
Cryopreservation
Cultivation
Diplonemids
Isolation
Large-scale cultivation

Access to Document

10.1007/978-1-0716-5142-1_2

Cite this

Tashyreva, D., Prokopchuk, G., Iorillo, O., Valach, M., Škodová-Sveráková, I., Shin, D. W., Letts, J. A., Burger, G., Lukeš, J., & Faktorová, D. (2026). Isolation and Cultivation of Diplonemids. In P. A. M. Michels, M. L. Ginger, A. Karnkowska, L.-I. McCall, & A. M. Silber (Eds.), Euglenozoa: Methods and Protocols (1st ed., Vol. 1, pp. 25-40). (Methods in Molecular Biology; Vol. 3013). Humana Press. https://doi.org/10.1007/978-1-0716-5142-1_2

@inbook{573417fdec3a45b49b348a0692e0b598,

title = "Isolation and Cultivation of Diplonemids",

abstract = "Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.",

keywords = "Axenization, Cryopreservation, Cultivation, Diplonemids, Isolation, Large-scale cultivation",

author = "Daria Tashyreva and Galina Prokopchuk and Orsola Iorillo and Matus Valach and Ingrid {\v S}kodov{\'a}-Sver{\'a}kov{\'a} and Shin, \{Dong Woo\} and Letts, \{James A.\} and Gertraud Burger and Julius Luke{\v s} and Drahom{\'i}ra Faktorov{\'a}",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.",

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doi = "10.1007/978-1-0716-5142-1\_2",

language = "English",

isbn = "9781071651414",

volume = "1",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "25--40",

editor = "Michels, \{Paul A.M.\} and Ginger, \{Michael L.\} and Anna Karnkowska and Laura-Isobel McCall and Silber, \{Ariel M.\}",

booktitle = "Euglenozoa",

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}

Tashyreva, D, Prokopchuk, G, Iorillo, O, Valach, M, Škodová-Sveráková, I, Shin, DW, Letts, JA, Burger, G, Lukeš, J & Faktorová, D 2026, Isolation and Cultivation of Diplonemids. in PAM Michels, ML Ginger, A Karnkowska, L-I McCall & AM Silber (eds), Euglenozoa: Methods and Protocols. 1st edn, vol. 1, Methods in Molecular Biology, vol. 3013, Humana Press, New York NY USA, pp. 25-40. https://doi.org/10.1007/978-1-0716-5142-1_2

Isolation and Cultivation of Diplonemids. / Tashyreva, Daria; Prokopchuk, Galina; Iorillo, Orsola et al.
Euglenozoa: Methods and Protocols. ed. / Paul A.M. Michels; Michael L. Ginger; Anna Karnkowska; Laura-Isobel McCall; Ariel M. Silber. Vol. 1 1st. ed. New York NY USA: Humana Press, 2026. p. 25-40 (Methods in Molecular Biology; Vol. 3013).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Isolation and Cultivation of Diplonemids

AU - Tashyreva, Daria

AU - Prokopchuk, Galina

AU - Iorillo, Orsola

AU - Valach, Matus

AU - Škodová-Sveráková, Ingrid

AU - Shin, Dong Woo

AU - Letts, James A.

AU - Burger, Gertraud

AU - Lukeš, Julius

AU - Faktorová, Drahomíra

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2026.

PY - 2026

Y1 - 2026

N2 - Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.

AB - Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.

KW - Axenization

KW - Cryopreservation

KW - Cultivation

KW - Diplonemids

KW - Isolation

KW - Large-scale cultivation

UR - https://www.scopus.com/pages/publications/105029324595

U2 - 10.1007/978-1-0716-5142-1_2

DO - 10.1007/978-1-0716-5142-1_2

M3 - Chapter (Book)

C2 - 41627728

AN - SCOPUS:105029324595

SN - 9781071651414

SN - 9781071651445

VL - 1

T3 - Methods in Molecular Biology

SP - 25

EP - 40

BT - Euglenozoa

A2 - Michels, Paul A.M.

A2 - Ginger, Michael L.

A2 - Karnkowska, Anna

A2 - McCall, Laura-Isobel

A2 - Silber, Ariel M.

PB - Humana Press

CY - New York NY USA

ER -

Isolation and Cultivation of Diplonemids

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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Cite this