Isolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies

Katie L. Smith, Joshua M. Hale, Jeremy J. Austin, Jane Melville

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3 Citations (Scopus)


Eight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was N a = 9.38 and N a = 7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.

Original languageEnglish
Pages (from-to)621-624
Number of pages4
JournalConservation Genetics Resources
Issue number4
Publication statusPublished - Oct 2011
Externally publishedYes


  • Amphibian
  • Hybrid zone
  • Hybridization
  • Litoria ewingii
  • Litoria paraewingi
  • Litoria verreauxii alpina
  • Microsatellite
  • South-eastern Australia
  • Species boundaries
  • Tree frog

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