Isolation and characterization of Actinobacteria with antibacterial activity from soil and rhizosphere soil

Adeline Su Yien Ting, Siew Hoon Tan, Mei Kay Wai

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In our study, we isolated 33 isolates of Actinobacteria which were identified into 16 different genera based on their cultural, morphological and biochemical characteristics. Both soil and rhizosphere soil samples yielded 17 and 16 isolates, which were grouped into nine and seven different genera, respectively. This showed that similar number of isolates can be recovered form both soil and rhizosphere samples. Both samples however hosted different genera of Actinobacteria with only one common genus found in both samples, Streptomyces spp. Thirty isolates produced antibacterial activity towards the tested bacterial pathogens (Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus). Twenty three of the isolates (10 from soil, 13 from rhizosphere oil) have antibacterial activity towards all three bacterial pathogens, establishing their broad-spectrum of antibacterial activity. Our study has shown that established antibiotic-producing Actinobacteria (Streptomyces, Actinomadura and Streptoverticillium) as well as uncommon antibiotic-producing genera (Dactylosporangium, Actinobispora, Promicromonospora, Saccharomonospora, Glycomyces, Saccharothrix, Nocardiopsis, Kitasatosporia and Microbispora) can be readily isolated from soil or/and rhizosphere samples and were effective towards the bacterial pathogens. These Actinobacteria isolates can be further investigated to identify the antibacterial compounds produced and their mechanisms of inhibition towards bacterial pathogens.

Original languageEnglish
Pages (from-to)4053-4059
Number of pages7
JournalAustralian Journal of Basic and Applied Sciences
Issue number4
Publication statusPublished - Oct 2009
Externally publishedYes


  • Actinobacteria
  • Antibacterial activity
  • Antibiotic-producing
  • Bacterial pathogens
  • Rhizosphere soil
  • Soil

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