Isolation and characterization of a myeloma-spleen-cell hybrid producing antibody to phenylalanine hydroxylase

R. G.H. Cotton; I. G. Jennings; K. H. Choo; K. Fowler

doi:10.1042/bj1910777

Isolation and characterization of a myeloma-spleen-cell hybrid producing antibody to phenylalanine hydroxylase

R. G.H. Cotton, I. G. Jennings, K. H. Choo, K. Fowler

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14 Citations (Scopus)

Abstract

Application of the technique of myeloma-spleen-cell fusion has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.

Original language	English
Pages (from-to)	777-783
Number of pages	7
Journal	Biochemical Journal
Volume	191
Issue number	3
DOIs	https://doi.org/10.1042/bj1910777
Publication status	Published - 1 Jan 1980
Externally published	Yes

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abstract = "Application of the technique of myeloma-spleen-cell fusion has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.",

author = "Cotton, \{R. G.H.\} and Jennings, \{I. G.\} and Choo, \{K. H.\} and K. Fowler",

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AU - Cotton, R. G.H.

AU - Jennings, I. G.

AU - Choo, K. H.

AU - Fowler, K.

PY - 1980/1/1

Y1 - 1980/1/1

N2 - Application of the technique of myeloma-spleen-cell fusion has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.

AB - Application of the technique of myeloma-spleen-cell fusion has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.

UR - https://www.scopus.com/pages/publications/0019133497

U2 - 10.1042/bj1910777

DO - 10.1042/bj1910777

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VL - 191

SP - 777

EP - 783

JO - Biochemical Journal

JF - Biochemical Journal

IS - 3

ER -

Isolation and characterization of a myeloma-spleen-cell hybrid producing antibody to phenylalanine hydroxylase

Abstract

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