Isolation and characterization of a myeloma-spleen-cell hybrid producing antibody to phenylalanine hydroxylase

R. G.H. Cotton, I. G. Jennings, K. H. Choo, K. Fowler

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Abstract

Application of the technique of myeloma-spleen-cell fusion has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.

Original languageEnglish
Pages (from-to)777-783
Number of pages7
JournalBiochemical Journal
Volume191
Issue number3
DOIs
Publication statusPublished - 1 Jan 1980
Externally publishedYes

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