TY - JOUR
T1 - Isolation and antioxidative properties of phenolics-saponins rich fraction from defatted rice bran
AU - Chan, Kim Wei
AU - Khong, Nicholas M.H.
AU - Iqbal, Shahid
AU - Ismail, Maznah
N1 - Funding Information:
The authors are thankful to Universiti Putra Malaysia as well as the Malaysian Ministry of Science, Technology and Innovation for providing funding for this work, respectively under the Research University Grant Scheme (RUGS) via Project No. 04-01-09-0609RU and the e-Science Fund via Project No. 02-01-04-SF1597. The authors also acknowledge the technical supports of the Institute of Bioscience, Universiti Putra Malaysia.
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/5
Y1 - 2013/5
N2 - The study presents a protocol for the preparation of phenolics-saponins rich fraction (PSRF), a new active nutraceutical from defatted rice bran followed by the determination of its antioxidant properties. PSRF was prepared by employing a simple alcoholic fractionation procedure on the crude alcoholic extract (CAE) of defatted rice bran. PSRF was found to be significantly higher in the contents of total phenolic, saponin, and steroidal saponin than CAE and its counterpart, aqueous fraction (AqF) (p < 0.05). Except for iron chelating activity, PSRF exhibited notably higher activity than CAE and AqF in all antioxidant activity assays performed (p < 0.05). HPLC-DAD analysis revealed that PSRF contained substantially higher amounts of gallic acid, 4-hydroxybenzoic acid, caffeic acid, p-coumaric acid, and ferulic acid than CAE and AqF (p < 0.05). In conclusion, alcoholic fractionation of CAE simultaneously concentrated the phenolic compounds and saponins into PSRF, thus contributed to its higher antioxidant activity. Due to its elevated antioxidant properties, PSRF may be recommended for investigation as an active ingredient in the nutraceutical, functional food, and natural food preservative formulations. This is also the first report suggesting defatted rice bran as a potential and sustainable source of saponins.
AB - The study presents a protocol for the preparation of phenolics-saponins rich fraction (PSRF), a new active nutraceutical from defatted rice bran followed by the determination of its antioxidant properties. PSRF was prepared by employing a simple alcoholic fractionation procedure on the crude alcoholic extract (CAE) of defatted rice bran. PSRF was found to be significantly higher in the contents of total phenolic, saponin, and steroidal saponin than CAE and its counterpart, aqueous fraction (AqF) (p < 0.05). Except for iron chelating activity, PSRF exhibited notably higher activity than CAE and AqF in all antioxidant activity assays performed (p < 0.05). HPLC-DAD analysis revealed that PSRF contained substantially higher amounts of gallic acid, 4-hydroxybenzoic acid, caffeic acid, p-coumaric acid, and ferulic acid than CAE and AqF (p < 0.05). In conclusion, alcoholic fractionation of CAE simultaneously concentrated the phenolic compounds and saponins into PSRF, thus contributed to its higher antioxidant activity. Due to its elevated antioxidant properties, PSRF may be recommended for investigation as an active ingredient in the nutraceutical, functional food, and natural food preservative formulations. This is also the first report suggesting defatted rice bran as a potential and sustainable source of saponins.
KW - Antioxidant activity
KW - Defatted rice bran
KW - Phenolic compounds
KW - Saponins
UR - http://www.scopus.com/inward/record.url?scp=84877128550&partnerID=8YFLogxK
U2 - 10.1016/j.jcs.2013.02.002
DO - 10.1016/j.jcs.2013.02.002
M3 - Article
AN - SCOPUS:84877128550
SN - 0733-5210
VL - 57
SP - 480
EP - 485
JO - Journal of Cereal Science
JF - Journal of Cereal Science
IS - 3
ER -