Isolation, amino acid sequence and action of guinea-pig ACTH on aldosterone production by glomerulosa cells

A. I. Smith, C. A. Wallace, R. L. Moritz, R. J. Simpson, L. B. Schmauk-White, E. A. Woodcock, J. W. Funder

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Abstract

Though minor sequence differences between-species have been reported for adrenocorticotrophin, ACTH(1-39), the steroidogenic moiety ACTH(1-24) has appeared invariant in mammals. We here report the isolation, purification and amino acid sequencing of guinea-pig (GP) ACTH in which Pro24 is replaced by Ala24, and the demonstration that GP-ACTH stimulates aldosterone production to maximal levels well above those seen with human ACTH(1-39) or Synacthen, ACTH(1-24) amide, the synthetic ACTH fragment widely used for diagnostic and therapeutic purposes.

Original languageEnglish
JournalJournal of Endocrinology
Volume115
Issue number2
DOIs
Publication statusPublished - 1 Jan 1987

Cite this

Smith, A. I. ; Wallace, C. A. ; Moritz, R. L. ; Simpson, R. J. ; Schmauk-White, L. B. ; Woodcock, E. A. ; Funder, J. W. / Isolation, amino acid sequence and action of guinea-pig ACTH on aldosterone production by glomerulosa cells. In: Journal of Endocrinology. 1987 ; Vol. 115, No. 2.
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Isolation, amino acid sequence and action of guinea-pig ACTH on aldosterone production by glomerulosa cells. / Smith, A. I.; Wallace, C. A.; Moritz, R. L.; Simpson, R. J.; Schmauk-White, L. B.; Woodcock, E. A.; Funder, J. W.

In: Journal of Endocrinology, Vol. 115, No. 2, 01.01.1987.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Isolation, amino acid sequence and action of guinea-pig ACTH on aldosterone production by glomerulosa cells

AU - Smith, A. I.

AU - Wallace, C. A.

AU - Moritz, R. L.

AU - Simpson, R. J.

AU - Schmauk-White, L. B.

AU - Woodcock, E. A.

AU - Funder, J. W.

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AB - Though minor sequence differences between-species have been reported for adrenocorticotrophin, ACTH(1-39), the steroidogenic moiety ACTH(1-24) has appeared invariant in mammals. We here report the isolation, purification and amino acid sequencing of guinea-pig (GP) ACTH in which Pro24 is replaced by Ala24, and the demonstration that GP-ACTH stimulates aldosterone production to maximal levels well above those seen with human ACTH(1-39) or Synacthen, ACTH(1-24) amide, the synthetic ACTH fragment widely used for diagnostic and therapeutic purposes.

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