Isolating valuable coffee diterpenes by using an inexpensive procedure

Fábio Junior Moreira Novaes, Fernanda Alves Lima, Verônica Calado, Philip John Marriott, Francisco Radler de Aquino Neto, Claudia Moraes Rezende

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Cafestol and kahweol (C&K) are natural coffee diterpenes with high commercial value. There is thus interest in their synthesis although their isolation from natural products remains an alternative to recovering high quantities of C&K. The present work aimed at developing an isolation procedure to obtain C&K for both analytical purposes and in gram-scale from coffee beans. The procedure was divided into three steps: an initial saponification reaction optimized by Factorial Design, a choice of solvent for extraction of a crude C&K mixture based on Hansen Solubility Parameters, and a subsequent purification by column chromatography and preparative liquid chromatography in recycling mode to improve C&K mixture purity and for separate preparative collection of each diterpene. The first two steps are useful to maximize yield for C&K quantification, and the complete procedure allowed isolation of 1.5 g of C&K per 300 g of green coffee beans. This isolation procedure can be repeated 25 times using ca. US$1k in reagents, with an estimated value of product varying from US$227k to US$322k for a diterpene mass of 37.5 g, at the prevailing commercial rate.

Original languageEnglish
Article number112494
Number of pages9
JournalIndustrial Crops and Products
Volume152
DOIs
Publication statusPublished - 15 Sep 2020

Keywords

  • Coffea arabica
  • Diterpenes cafestol and kahweol
  • Experimental design
  • Maximize preparative diterpene isolation
  • Natural product scale-up

Cite this

Novaes, F. J. M., Lima, F. A., Calado, V., Marriott, P. J., de Aquino Neto, F. R., & Rezende, C. M. (2020). Isolating valuable coffee diterpenes by using an inexpensive procedure. Industrial Crops and Products, 152, [112494]. https://doi.org/10.1016/j.indcrop.2020.112494