IR and Raman imaging of murine brains from control and ApoE/LDLR-/- mice with advanced atherosclerosis

Kamila Kochan; Karolina Chrabaszcz; Barbara Szczur; Edyta Maslak; Jakub Dybas; Katarzyna M. Marzec

doi:10.1039/c6an00107f

IR and Raman imaging of murine brains from control and ApoE/LDLR^-/- mice with advanced atherosclerosis

Kamila Kochan, Karolina Chrabaszcz, Barbara Szczur, Edyta Maslak, Jakub Dybas, Katarzyna M. Marzec

Research output: Contribution to journal › Article › Research › peer-review

23 Citations (Scopus)

Abstract

Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR^-/- mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.

Original language	English
Pages (from-to)	5329-5338
Number of pages	10
Journal	Analyst
Volume	141
Issue number	18
DOIs	https://doi.org/10.1039/c6an00107f
Publication status	Published - 21 Sept 2016
Externally published	Yes

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abstract = "Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR-/- mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.",

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AU - Kochan, Kamila

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AU - Maslak, Edyta

AU - Dybas, Jakub

AU - Marzec, Katarzyna M.

PY - 2016/9/21

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N2 - Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR-/- mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.

AB - Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR-/- mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.

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IR and Raman imaging of murine brains from control and ApoE/LDLR-/- mice with advanced atherosclerosis

Abstract

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IR and Raman imaging of murine brains from control and ApoE/LDLR^-/- mice with advanced atherosclerosis