IR and Raman imaging of murine brains from control and ApoE/LDLR-/- mice with advanced atherosclerosis

Kamila Kochan, Karolina Chrabaszcz, Barbara Szczur, Edyta Maslak, Jakub Dybas, Katarzyna M. Marzec

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18 Citations (Scopus)

Abstract

Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR-/- mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.

Original languageEnglish
Pages (from-to)5329-5338
Number of pages10
JournalAnalyst
Volume141
Issue number18
DOIs
Publication statusPublished - 21 Sep 2016
Externally publishedYes

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