Ionic regulation of the binding of dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid to synaptosome-enriched homogenates of rat cerebral cortex

David T. Manallack, Philip M. Beart

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The ionic requirements of the site labelled by dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid ([3H]dl-2AP7) in synaptosome-enriched homogenates of rat cerebral cortex were examined using radioligand binding methodology. Binding of [3H]dl-2AP7 was increased by calcium and chloride ions by an apparently non-competitive mechanism. The actions of ions on specific binding displayed similarities to the effects of ions on dl-[3H]2-amino-4-phosphonobutyric acid and Cl /Ca2+-dependent l-[3H]glutamate ([3H]Glu) binding sites but were more consistent with the Glu-C binding site.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalNeuroscience Letters
Issue number1
Publication statusPublished - 11 Jul 1986
Externally publishedYes


  • 2-amino-7-phosphono-heptanoate
  • cation
  • chloride
  • divalent
  • glutamate transmitter
  • N-methyl-d-aspartate
  • receptor

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