Ionic regulation of the binding of dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid to synaptosome-enriched homogenates of rat cerebral cortex

David T. Manallack; Philip M. Beart

doi:10.1016/0304-3940(86)90221-1

Ionic regulation of the binding of dl-2-amino-7-phosphono-[4,5-³H]heptanoic acid to synaptosome-enriched homogenates of rat cerebral cortex

David T. Manallack, Philip M. Beart

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The ionic requirements of the site labelled by dl-2-amino-7-phosphono-[4,5-³H]heptanoic acid ([³H]dl-2AP7) in synaptosome-enriched homogenates of rat cerebral cortex were examined using radioligand binding methodology. Binding of [³H]dl-2AP7 was increased by calcium and chloride ions by an apparently non-competitive mechanism. The actions of ions on specific binding displayed similarities to the effects of ions on dl-[³H]2-amino-4-phosphonobutyric acid and Cl /Ca²⁺-dependent l-[³H]glutamate ([³H]Glu) binding sites but were more consistent with the Glu-C binding site.

Original language	English
Pages (from-to)	11-16
Number of pages	6
Journal	Neuroscience Letters
Volume	68
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(86)90221-1
Publication status	Published - 11 Jul 1986
Externally published	Yes

Keywords

2-amino-7-phosphono-heptanoate
cation
chloride
divalent
glutamate transmitter
N-methyl-d-aspartate
receptor

Access to Document

10.1016/0304-3940(86)90221-1

Cite this

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title = "Ionic regulation of the binding of dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid to synaptosome-enriched homogenates of rat cerebral cortex",

abstract = "The ionic requirements of the site labelled by dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid ([3H]dl-2AP7) in synaptosome-enriched homogenates of rat cerebral cortex were examined using radioligand binding methodology. Binding of [3H]dl-2AP7 was increased by calcium and chloride ions by an apparently non-competitive mechanism. The actions of ions on specific binding displayed similarities to the effects of ions on dl-[3H]2-amino-4-phosphonobutyric acid and Cl /Ca2+-dependent l-[3H]glutamate ([3H]Glu) binding sites but were more consistent with the Glu-C binding site.",

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T1 - Ionic regulation of the binding of dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid to synaptosome-enriched homogenates of rat cerebral cortex

AU - Manallack, David T.

AU - Beart, Philip M.

PY - 1986/7/11

Y1 - 1986/7/11

N2 - The ionic requirements of the site labelled by dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid ([3H]dl-2AP7) in synaptosome-enriched homogenates of rat cerebral cortex were examined using radioligand binding methodology. Binding of [3H]dl-2AP7 was increased by calcium and chloride ions by an apparently non-competitive mechanism. The actions of ions on specific binding displayed similarities to the effects of ions on dl-[3H]2-amino-4-phosphonobutyric acid and Cl /Ca2+-dependent l-[3H]glutamate ([3H]Glu) binding sites but were more consistent with the Glu-C binding site.

AB - The ionic requirements of the site labelled by dl-2-amino-7-phosphono-[4,5-3H]heptanoic acid ([3H]dl-2AP7) in synaptosome-enriched homogenates of rat cerebral cortex were examined using radioligand binding methodology. Binding of [3H]dl-2AP7 was increased by calcium and chloride ions by an apparently non-competitive mechanism. The actions of ions on specific binding displayed similarities to the effects of ions on dl-[3H]2-amino-4-phosphonobutyric acid and Cl /Ca2+-dependent l-[3H]glutamate ([3H]Glu) binding sites but were more consistent with the Glu-C binding site.

KW - 2-amino-7-phosphono-heptanoate

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KW - chloride

KW - divalent

KW - glutamate transmitter

KW - N-methyl-d-aspartate

KW - receptor

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