Ionic liquid phases with comprehensive two-dimensional gas chromatography of fatty acid methyl esters

Siriluck Pojjanapornpun, Yada Nolvachai, Kornkanok Aryusuk, Chadin Kulsing, Kanit Krisnangkura, Philip J. Marriott

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (TM), programmable TM during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets. Although IL phases are classified as polar, relative polarity of the two phases comprising individual GC × GC column sets permits combination of less-polar IL/polar IL and polar IL/less-polar IL column sets; it was observed that a polar/less-polar column set provided better separation of FAME. A higher first dimension (1D) phase polarity combined with a lower 2D phase polarity, for instance 1D IL111i with 2D IL59 gave the best result; the greater difference in 1D/2D phase polarity results in increasing occupancy of peak area in the 2D space. The IL111i/IL59 column set was selected for analysis of fatty acids in fat and oil products (butter, margarine, fish oil and canola oil). Compared with the conventional IL111, IL111i showed reduced column bleed which makes this more suited to GC × GC analysis of FAME. The proposed method offers a fast profiling approach with good repeatability of analysis of FAME.

Original languageEnglish
Pages (from-to)4669–4677
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume410
Issue number19
DOIs
Publication statusPublished - Jul 2018

Keywords

  • Comprehensive two-dimensional gas chromatography
  • Fatty acid methyl ester
  • Inert IL phases
  • Ionic liquids
  • Solid-state modulation

Cite this

Pojjanapornpun, Siriluck ; Nolvachai, Yada ; Aryusuk, Kornkanok ; Kulsing, Chadin ; Krisnangkura, Kanit ; Marriott, Philip J. / Ionic liquid phases with comprehensive two-dimensional gas chromatography of fatty acid methyl esters. In: Analytical and Bioanalytical Chemistry. 2018 ; Vol. 410, No. 19. pp. 4669–4677.
@article{0b3347fbed9b46a882f0794ec4435ad1,
title = "Ionic liquid phases with comprehensive two-dimensional gas chromatography of fatty acid methyl esters",
abstract = "New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (TM), programmable TM during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets. Although IL phases are classified as polar, relative polarity of the two phases comprising individual GC × GC column sets permits combination of less-polar IL/polar IL and polar IL/less-polar IL column sets; it was observed that a polar/less-polar column set provided better separation of FAME. A higher first dimension (1D) phase polarity combined with a lower 2D phase polarity, for instance 1D IL111i with 2D IL59 gave the best result; the greater difference in 1D/2D phase polarity results in increasing occupancy of peak area in the 2D space. The IL111i/IL59 column set was selected for analysis of fatty acids in fat and oil products (butter, margarine, fish oil and canola oil). Compared with the conventional IL111, IL111i showed reduced column bleed which makes this more suited to GC × GC analysis of FAME. The proposed method offers a fast profiling approach with good repeatability of analysis of FAME.",
keywords = "Comprehensive two-dimensional gas chromatography, Fatty acid methyl ester, Inert IL phases, Ionic liquids, Solid-state modulation",
author = "Siriluck Pojjanapornpun and Yada Nolvachai and Kornkanok Aryusuk and Chadin Kulsing and Kanit Krisnangkura and Marriott, {Philip J.}",
year = "2018",
month = "7",
doi = "10.1007/s00216-018-0944-7",
language = "English",
volume = "410",
pages = "4669–4677",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer-Verlag London Ltd.",
number = "19",

}

Ionic liquid phases with comprehensive two-dimensional gas chromatography of fatty acid methyl esters. / Pojjanapornpun, Siriluck; Nolvachai, Yada; Aryusuk, Kornkanok; Kulsing, Chadin; Krisnangkura, Kanit; Marriott, Philip J.

In: Analytical and Bioanalytical Chemistry, Vol. 410, No. 19, 07.2018, p. 4669–4677.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Ionic liquid phases with comprehensive two-dimensional gas chromatography of fatty acid methyl esters

AU - Pojjanapornpun, Siriluck

AU - Nolvachai, Yada

AU - Aryusuk, Kornkanok

AU - Kulsing, Chadin

AU - Krisnangkura, Kanit

AU - Marriott, Philip J.

PY - 2018/7

Y1 - 2018/7

N2 - New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (TM), programmable TM during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets. Although IL phases are classified as polar, relative polarity of the two phases comprising individual GC × GC column sets permits combination of less-polar IL/polar IL and polar IL/less-polar IL column sets; it was observed that a polar/less-polar column set provided better separation of FAME. A higher first dimension (1D) phase polarity combined with a lower 2D phase polarity, for instance 1D IL111i with 2D IL59 gave the best result; the greater difference in 1D/2D phase polarity results in increasing occupancy of peak area in the 2D space. The IL111i/IL59 column set was selected for analysis of fatty acids in fat and oil products (butter, margarine, fish oil and canola oil). Compared with the conventional IL111, IL111i showed reduced column bleed which makes this more suited to GC × GC analysis of FAME. The proposed method offers a fast profiling approach with good repeatability of analysis of FAME.

AB - New generation inert ionic liquid (iIL) GC columns IL60i, IL76i and IL111i, comprising phosphonium or imidazolium cationic species, were investigated for separation of fatty acid methyl esters (FAME). In general, the iIL phases provide comparable retention times to their corresponding conventional columns, with only minor selectivity differences. The average tailing factors and peak widths were noticeably improved (reduced) for IL60i and IL76i, while they were slightly improved for IL111i. Inert IL phase columns were coupled with conventional IL columns in comprehensive two-dimensional GC (GC × GC) with a solid-state modulator which offers variable modulation temperature (TM), programmable TM during analysis and trapping stationary phase material during the trap/release (modulation) process, independent of oven T and column sets. Although IL phases are classified as polar, relative polarity of the two phases comprising individual GC × GC column sets permits combination of less-polar IL/polar IL and polar IL/less-polar IL column sets; it was observed that a polar/less-polar column set provided better separation of FAME. A higher first dimension (1D) phase polarity combined with a lower 2D phase polarity, for instance 1D IL111i with 2D IL59 gave the best result; the greater difference in 1D/2D phase polarity results in increasing occupancy of peak area in the 2D space. The IL111i/IL59 column set was selected for analysis of fatty acids in fat and oil products (butter, margarine, fish oil and canola oil). Compared with the conventional IL111, IL111i showed reduced column bleed which makes this more suited to GC × GC analysis of FAME. The proposed method offers a fast profiling approach with good repeatability of analysis of FAME.

KW - Comprehensive two-dimensional gas chromatography

KW - Fatty acid methyl ester

KW - Inert IL phases

KW - Ionic liquids

KW - Solid-state modulation

UR - http://www.scopus.com/inward/record.url?scp=85042132726&partnerID=8YFLogxK

U2 - 10.1007/s00216-018-0944-7

DO - 10.1007/s00216-018-0944-7

M3 - Article

AN - SCOPUS:85042132726

VL - 410

SP - 4669

EP - 4677

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 19

ER -