Investigation of phytochemical composition and enzyme inhibitory potential of Anagallis arvensis L.

Hammad Saleem, Gokhan Zengin, Marcello Locatelli, Syafiq Asnawi Zainal Abidin, Nafees Ahemad

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2 Citations (Scopus)


Anagallis arvensis L. commonly known as ‘Scarlet Pimpernel’ has been used in folklore as natural remedy for treating common ailments. The present research is aimed to explore the phytochemical composition and enzyme inhibition potential of methanol and dichloromethane (DCM) extracts of A. arvensis aerial and root parts. The phytochemical composition was established via HPLC-PDA polyphenolic quantification and UHPLC-MS analysis, while the inhibition potential against amylase and tyrosinase enzymes were assessed using standard in vitro protocols. The HPLC-PDA polyphenolic quantification revealed the presence of important compounds including catechin, gallic acid, chlorogenic acid, and ferulic acid, whereas 34 different secondary metabolites were tentatively identified by UHPLC-MS of both the DCM extracts. All the extracts showed moderate tyrosinase and a weak amylase inhibition activity. The aerial-DCM extract showed comparatively higher tyrosinase and amylase enzyme inhibition potential, which may be due to the presence of secondary metabolites as tentatively identified by its UHPLC-MS profiling.

Original languageEnglish
Pages (from-to)3750-3755
Number of pages6
JournalNatural Product Research
Issue number14
Publication statusPublished - 15 Jul 2022


  • amylase
  • Anagallis arvensis
  • HPLC
  • phenolic
  • secondary metabolites
  • tyrosinase

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