Investigation of molecular transfer processes across phospholipid monolayers by the combined scanning electrochemical microscopy-langmuir trough technique

Jorg Strutwolf, Jie Zhang, Patrick R Unwin

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Scanning electrochemical microscopy (SECM) has emerged as a powerful technique for inducing and monitoring molecular transfer processes across water/air and liquid/liquid interfaces. At the same time, the Langmuir trough technique is a well established method for controlling the lateral pressure of molecular films of amphiphilic molecules at interfaces. A combination of both methods allows the investigation of the permeability of monolayers in a defined state. A brief introduction of the SECM technique and the experimental set-up is presented. The application of the combined SECM-Langmuir trough technique to measure passive diffusion of small molecules (O-2 and Br-2) across phospholipid monolayers is then reviewed. Phospholipid monolayers at liquid/liquid and liquid/air interfaces serve as simple biomimetic models for biomembranes and the results of the combined SECM-Langmuir trough measurements have implications for understanding passive diffusion across cellular membranes.
Original languageEnglish
Pages (from-to)195 - 217
Number of pages23
JournalProgress in Reaction Kinetics and Mechanism
Issue number4
Publication statusPublished - 2007
Externally publishedYes

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