Investigation of glycopolymer-lectin interactions using QCM-d: Comparison of surface binding with inhibitory activity

Yanzi Gou, Sarah Jane Richards, David M. Haddleton, Matthew I. Gibson

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Glycopolymers offer many opportunities for interfacing synthetic materials with biological systems. However, the nature of the interactions between glycopolymers and their biological targets, lectins, and the structural features necessary to obtain high-affinity materials are not fully understood. Here, the enhancement in binding affinity of multivalent glycopolymers to their corresponding lectins is investigated by quartz-crystal microbalance with dissipation monitoring (QCM-d). This technique allows the conformation of the adsorbed polymers to be probed and the direct observation of spanning of multiple binding sites on lectin-functional surfaces. The measured affinity was compared to the anti-adhesion activity of the polymers in solution, and it is shown that increased association constants did not directly correlate with inhibitory activity.

Original languageEnglish
Pages (from-to)1634-1640
Number of pages7
JournalPolymer Chemistry
Volume3
Issue number6
DOIs
Publication statusPublished - 2012
Externally publishedYes

Cite this