Investigating the role of RIO protein kinases in Caenorhabditis elegans

Tasha K Mendes, Stevan Novakovic, Greta C Raymant, Sonja E Bertram, Reza Esmaillie, Saravanapriah Nadarajan, Bert Breugelmans, Andreas Hofmann, Robin B Gasser, Monica P Colaiacovo, Peter R Boag

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Abstract

RIO protein kinases (RIOKs) are a relatively conserved family of enzymes implicated in cell cycle control and ribosomal RNA processing. Despite their functional importance, they remain a poorly understood group of kinases in multicellular organisms. Here, we show that the C. elegans genome contains one member of each of the three RIOK sub-families and that each of the genes coding for them has a unique tissue expression pattern. Our analysis showed that the gene encoding RIOK-1 (riok-1) was broadly and strongly expressed. Interestingly, the intestinal expression of riok-1 was dependent upon two putative binding sites for the oxidative and xenobiotic stress response transcription factor SKN-1. RNA interference (RNAi)-mediated knock down of riok-1 resulted in germline defects, including defects in germ line stem cell proliferation, oocyte maturation and the production of endomitotic oocytes. Taken together, our findings indicate new functions for RIOK-1 in post mitotic tissues and in reproduction.
Original languageEnglish
Article numbere0117444
Number of pages17
JournalPLoS ONE
Volume10
Issue number2
DOIs
Publication statusPublished - 2015

Cite this

Mendes, T. K., Novakovic, S., Raymant, G. C., Bertram, S. E., Esmaillie, R., Nadarajan, S., Breugelmans, B., Hofmann, A., Gasser, R. B., Colaiacovo, M. P., & Boag, P. R. (2015). Investigating the role of RIO protein kinases in Caenorhabditis elegans. PLoS ONE, 10(2), [e0117444]. https://doi.org/10.1371/journal.pone.0117444