Investigating structural and biochemical correlates of ganglion cell dysfunction in streptozotocin-induced diabetic rats

Bang Bui, Michelle Loeliger, Merlin Thomas, Algis Vingrys, Sandra Rees, Christine Nguyen, Zheng He, Mary Tolcos

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34 Citations (Scopus)

Abstract

The aim of this study was to determine whether inner retinal dysfunction in diabetic rats is correlated with structural and/or biochemical changes in the retina and optic nerve. Using the electroretinogram (ERG; -5.83 to 1.28 log cd.s.m(-2)) retinal function (photoreceptor, bipolar, amacrine and ganglion cell components) was measured in control (n=13; citrate buffer) and diabetic (n=13; streptozotocin, STZ, 50 mg kg(-1)) rats, 12 weeks following treatment. Retinae and optic nerves were analyzed for structural changes and retinae were assessed for alterations in growth factor/cytokine expression using quantitative real-time PCR. We found that phototransduction efficiency was reduced 12 weeks after STZ-induced diabetes (-30 ), leading to reduced amplitude of ON-bipolar (-18 ) and amacrine cell (-29 ) dominated responses; ganglion cell dysfunction (-84 ) was more profound. In the optic nerve, nerve fascicle area and myelin sheath thickness were reduced (p
Original languageEnglish
Pages (from-to)1076 - 1083
Number of pages8
JournalExperimental Eye Research
Volume88
Issue number6
DOIs
Publication statusPublished - 2009
Externally publishedYes

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