Investigating domestic shower settings as a risk factor for Acanthamoeba keratitis

Binod Rayamajhee, Dinesh Subedi, Seoyoung Won, Jamie Kim, Ajay Vijay, Jacqueline Tan, Fiona L. Henriquez, Mark Willcox, Nicole A. Carnt

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4 Citations (Scopus)


Acanthamoeba keratitis (AK) is a sight-threatening infection of the cornea, which is caused by soil and the waterborne protist Acanthamoeba spp. AK most commonly occurs during contact lens (CL) wear. Risk factors for AK have been linked to non-optimal lens hygiene practices and Acanthamoeba contamination of domestic water. This study investigated the prevalence of Acanthamoeba species in domestic showers in the greater Sydney region, as well as the perception of water contamination of CL as being a risk factor for AK among previous AK patients and their family and friends. Samples from four locations of 13 participants’ shower areas were cultured and Acanthamoeba 18S rRNA was amplified by PCR, followed by sequencing. Twenty-six responses were received to the online questionnaire. Fifteen water samples (29%, 15/52) contained amoeba that were morphologically classified as Acanthamoeba spp. PCR amplification confirmed the presence of Acanthamoeba spp. in four samples (8%, 4/52). Three isolates belonged to the T4, and one isolate to the T3 genotype. On the questionnaire survey, 96% (25/26) of respondents believed that water contamination was likely to be a risk factor for Acanthamoeba keratitis and 58% rated showering with CL as ‘extremely likely’ to be a risk factor for AK. Acanthamoeba pathogenic genotypes (T3 and T4) in the domestic bathroom water suggest that clinicians should remain vigilant in educating CL wearers about avoiding domestic water contamination of CL.

Original languageEnglish
Article number3493
Number of pages12
Issue number12
Publication statusPublished - 11 Dec 2020


  • Acanthamoeba keratitis
  • Contact lens
  • Domestic bathroom water contamination
  • Domestic shower
  • Prevalence
  • Risk factors

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