Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5

E. M. Menkhorst, M. L. Van Sinderen, K. Rainczuk, C. Cuman, A. Winship, E. Dimitriadis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.

Original languageEnglish
Article number8690
Number of pages11
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

Cite this

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title = "Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5",
abstract = "During the establishment of pregnancy, extravillous trophoblast (EVT) must invade into the uterine decidua to facilitate decidual artery remodelling to create the placental blood supply. The local decidual environment is thought to regulate trophoblast invasion, however these interactions are poorly defined in humans. Recent evidence in women suggests impaired decidualization is associated with miscarriage and preeclampsia. Primary human endometrial stromal cells (HESC) and first trimester extravillous trophoblast (EVTs) were used to assess the effect of EVT-secreted factors on HESC decidualization, adhesion, proliferation and migration. We determined the role of profilin (PFN)1, an EVT-secreted factor, on HESC function and identified a downstream target of PFN1. EVT-secreted factors induced HESC decidualization and enhanced decidualized HESC adhesion, proliferation and migration. Recombinant PFN1 enhanced methoxyprogesterone acetate-induced HESC decidualization and proliferation. PFN1 down-regulated the expression of lipoxygenase arachidonate 5-lipoxygenase (ALOX5) in HESC and THP-1 macrophages. ALOX5 localised to decidual cells and CD68+macrophages in 1st trimester decidua. This study demonstrated that EVT secretions, including PFN1, enhanced HESC decidualization and motility. This study has identified a new pathway that facilitates appropriate decidualization during the establishment of pregnancy.",
author = "Menkhorst, {E. M.} and {Van Sinderen}, {M. L.} and K. Rainczuk and C. Cuman and A. Winship and E. Dimitriadis",
year = "2017",
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Invasive trophoblast promote stromal fibroblast decidualization via Profilin 1 and ALOX5. / Menkhorst, E. M.; Van Sinderen, M. L.; Rainczuk, K.; Cuman, C.; Winship, A.; Dimitriadis, E.

In: Scientific Reports, Vol. 7, No. 1, 8690, 01.12.2017.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Menkhorst, E. M.

AU - Van Sinderen, M. L.

AU - Rainczuk, K.

AU - Cuman, C.

AU - Winship, A.

AU - Dimitriadis, E.

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