Invariant natural killer T cells shape the gut microbiota and regulate neutrophil recruitment and function during intestinal inflammation

Sj Shen, Kathryn Prame Kumar, Dragana Stanley, Robert J. Moore, Thi Thu Hao Van, Shu Wen Wen, Michael J. Hickey, Connie H.Y. Wong

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Invariant natural killer T (iNKT) cells and neutrophils play an increasingly important part in the pathogenesis of inflammatory diseases, but their precise roles in modulating colitis remain unclear. Previous studies have shown important interplays between host immune system and the gut microbiota, and the resulting modulation of inflammation. However, the interactions between iNKT cells, neutrophil and gut microbiota in regulating colitis pathology are poorly understood. Here, we show iNKT cell-deficient Jα18-/- mice display reduced dextran sodium sulfate (DSS)-induced colonic inflammation compared to their wild-type (WT) counterparts. We reveal that there is a distinct gut microbiota shaped by the absence of iNKT cells, which comprises of microorganisms that are associated with protection from colonic inflammation. Additionally, the reduced inflammation in Jα18-/- mice was correlated with increased expressions of neutrophil chemoattractant (Cxcl1 and Cxcl2) and increased neutrophil recruitment. However, these neutrophils were recruited to the colon at day 3 of our model, prior to observable clinical signs at day 5. Further analysis shows that these neutrophils, primed by the microbiota shaped by the lack of iNKT cells, exhibit anti-inflammatory and immune-modulatory properties. Indeed, depletion of neutrophils in DSS-treated Jα18-/- mice demonstrates that neutrophils confer an anti-colitogenic effect in the absence of iNKT cells. Thus, our data supports a changing dogma that neutrophils possess important regulatory roles in inflammation and highlights the complexity of the iNKT cell-microbiota-neutrophil axis in regulating colonic inflammation.

Original languageEnglish
Article number999
Number of pages15
JournalFrontiers in Immunology
Volume9
DOIs
Publication statusPublished - 7 May 2018

Keywords

  • Colitis
  • Inflammation
  • Innate immunity
  • Mucosal immunology
  • Neutrophils

Cite this

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title = "Invariant natural killer T cells shape the gut microbiota and regulate neutrophil recruitment and function during intestinal inflammation",
abstract = "Invariant natural killer T (iNKT) cells and neutrophils play an increasingly important part in the pathogenesis of inflammatory diseases, but their precise roles in modulating colitis remain unclear. Previous studies have shown important interplays between host immune system and the gut microbiota, and the resulting modulation of inflammation. However, the interactions between iNKT cells, neutrophil and gut microbiota in regulating colitis pathology are poorly understood. Here, we show iNKT cell-deficient Jα18-/- mice display reduced dextran sodium sulfate (DSS)-induced colonic inflammation compared to their wild-type (WT) counterparts. We reveal that there is a distinct gut microbiota shaped by the absence of iNKT cells, which comprises of microorganisms that are associated with protection from colonic inflammation. Additionally, the reduced inflammation in Jα18-/- mice was correlated with increased expressions of neutrophil chemoattractant (Cxcl1 and Cxcl2) and increased neutrophil recruitment. However, these neutrophils were recruited to the colon at day 3 of our model, prior to observable clinical signs at day 5. Further analysis shows that these neutrophils, primed by the microbiota shaped by the lack of iNKT cells, exhibit anti-inflammatory and immune-modulatory properties. Indeed, depletion of neutrophils in DSS-treated Jα18-/- mice demonstrates that neutrophils confer an anti-colitogenic effect in the absence of iNKT cells. Thus, our data supports a changing dogma that neutrophils possess important regulatory roles in inflammation and highlights the complexity of the iNKT cell-microbiota-neutrophil axis in regulating colonic inflammation.",
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Invariant natural killer T cells shape the gut microbiota and regulate neutrophil recruitment and function during intestinal inflammation. / Shen, Sj; Kumar, Kathryn Prame; Stanley, Dragana; Moore, Robert J.; Hao Van, Thi Thu; Wen, Shu Wen; Hickey, Michael J.; Wong, Connie H.Y.

In: Frontiers in Immunology, Vol. 9, 999, 07.05.2018.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Shen, Sj

AU - Kumar, Kathryn Prame

AU - Stanley, Dragana

AU - Moore, Robert J.

AU - Hao Van, Thi Thu

AU - Wen, Shu Wen

AU - Hickey, Michael J.

AU - Wong, Connie H.Y.

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