Intrauterine inflammation causes pulmonary hypertension and cardiovascular sequelae in preterm lambs

Graeme Polglase, Stuart Brian Hooper, Andrew W Gill, Beth Joyce Allison, Kelly Jane Crossley, Timothy James Murugesan Moss, Ilias Nitsos, Jane Jane Pillow, Martin Kluckow

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32 Citations (Scopus)

Abstract

Chorioamnionitis increases the risk and severity of persistent pulmonary hypertension of the newborn (PPHN) in preterm infants. Exposure of preterm fetal lambs to intra-amniotic (IA) lipopolysaccharide (LPS) induces chorioamnionitis, causes hypertrophy of pulmonary resistance arterioles and altered expression of pulmonary vascular growth proteins. We investigated the cardiopulmonary and systemic hemodynamic consequences of IA LPS in preterm lambs. Pregnant ewes received IA injection of LPS (n=6) or saline (controls; n=8)at 122 d gestation, 7 d prior to exteriorization, instrumentation and delivery of the fetus with pulmonary and systemic flow-probes and catheters at 129 d gestation. Newborn lambs were ventilated targeting a tidal volume of 6-7 mL/kg and a PEEP of 4 cmH(2)O. At 30 min all lambs underwent a PEEP challenge: PEEP was increased by 2 cmH(2)O at 10 min intervals to 10 cmH(2)O then decreased similarly to 4 cmH(2)O. Ventilation parameters, arterial blood flows and pressures were recorded in real-time for 90 min. LPS lambs had higher total protein in bronchoalveolar lavage fluid (p
Original languageEnglish
Pages (from-to)1757 - 1765
Number of pages9
JournalJournal of Applied Physiology
Volume108
Issue number6
DOIs
Publication statusPublished - 2010

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