Intrauterine inflammation causes pulmonary hypertension and cardiovascular sequelae in preterm lambs

Graeme Polglase, Stuart Brian Hooper, Andrew W Gill, Beth Joyce Allison, Kelly Jane Crossley, Timothy James Murugesan Moss, Ilias Nitsos, Jane Jane Pillow, Martin Kluckow

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Chorioamnionitis increases the risk and severity of persistent pulmonary hypertension of the newborn (PPHN) in preterm infants. Exposure of preterm fetal lambs to intra-amniotic (IA) lipopolysaccharide (LPS) induces chorioamnionitis, causes hypertrophy of pulmonary resistance arterioles and altered expression of pulmonary vascular growth proteins. We investigated the cardiopulmonary and systemic hemodynamic consequences of IA LPS in preterm lambs. Pregnant ewes received IA injection of LPS (n=6) or saline (controls; n=8)at 122 d gestation, 7 d prior to exteriorization, instrumentation and delivery of the fetus with pulmonary and systemic flow-probes and catheters at 129 d gestation. Newborn lambs were ventilated targeting a tidal volume of 6-7 mL/kg and a PEEP of 4 cmH(2)O. At 30 min all lambs underwent a PEEP challenge: PEEP was increased by 2 cmH(2)O at 10 min intervals to 10 cmH(2)O then decreased similarly to 4 cmH(2)O. Ventilation parameters, arterial blood flows and pressures were recorded in real-time for 90 min. LPS lambs had higher total protein in bronchoalveolar lavage fluid (p
Original languageEnglish
Pages (from-to)1757 - 1765
Number of pages9
JournalJournal of Applied Physiology
Volume108
Issue number6
DOIs
Publication statusPublished - 2010

Cite this

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title = "Intrauterine inflammation causes pulmonary hypertension and cardiovascular sequelae in preterm lambs",
abstract = "Chorioamnionitis increases the risk and severity of persistent pulmonary hypertension of the newborn (PPHN) in preterm infants. Exposure of preterm fetal lambs to intra-amniotic (IA) lipopolysaccharide (LPS) induces chorioamnionitis, causes hypertrophy of pulmonary resistance arterioles and altered expression of pulmonary vascular growth proteins. We investigated the cardiopulmonary and systemic hemodynamic consequences of IA LPS in preterm lambs. Pregnant ewes received IA injection of LPS (n=6) or saline (controls; n=8)at 122 d gestation, 7 d prior to exteriorization, instrumentation and delivery of the fetus with pulmonary and systemic flow-probes and catheters at 129 d gestation. Newborn lambs were ventilated targeting a tidal volume of 6-7 mL/kg and a PEEP of 4 cmH(2)O. At 30 min all lambs underwent a PEEP challenge: PEEP was increased by 2 cmH(2)O at 10 min intervals to 10 cmH(2)O then decreased similarly to 4 cmH(2)O. Ventilation parameters, arterial blood flows and pressures were recorded in real-time for 90 min. LPS lambs had higher total protein in bronchoalveolar lavage fluid (p",
author = "Graeme Polglase and Hooper, {Stuart Brian} and Gill, {Andrew W} and Allison, {Beth Joyce} and Crossley, {Kelly Jane} and Moss, {Timothy James Murugesan} and Ilias Nitsos and Pillow, {Jane Jane} and Martin Kluckow",
year = "2010",
doi = "10.1152/japplphysiol.01336.2009",
language = "English",
volume = "108",
pages = "1757 -- 1765",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "6",

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Intrauterine inflammation causes pulmonary hypertension and cardiovascular sequelae in preterm lambs. / Polglase, Graeme; Hooper, Stuart Brian; Gill, Andrew W; Allison, Beth Joyce; Crossley, Kelly Jane; Moss, Timothy James Murugesan; Nitsos, Ilias; Pillow, Jane Jane; Kluckow, Martin.

In: Journal of Applied Physiology, Vol. 108, No. 6, 2010, p. 1757 - 1765.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Intrauterine inflammation causes pulmonary hypertension and cardiovascular sequelae in preterm lambs

AU - Polglase, Graeme

AU - Hooper, Stuart Brian

AU - Gill, Andrew W

AU - Allison, Beth Joyce

AU - Crossley, Kelly Jane

AU - Moss, Timothy James Murugesan

AU - Nitsos, Ilias

AU - Pillow, Jane Jane

AU - Kluckow, Martin

PY - 2010

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N2 - Chorioamnionitis increases the risk and severity of persistent pulmonary hypertension of the newborn (PPHN) in preterm infants. Exposure of preterm fetal lambs to intra-amniotic (IA) lipopolysaccharide (LPS) induces chorioamnionitis, causes hypertrophy of pulmonary resistance arterioles and altered expression of pulmonary vascular growth proteins. We investigated the cardiopulmonary and systemic hemodynamic consequences of IA LPS in preterm lambs. Pregnant ewes received IA injection of LPS (n=6) or saline (controls; n=8)at 122 d gestation, 7 d prior to exteriorization, instrumentation and delivery of the fetus with pulmonary and systemic flow-probes and catheters at 129 d gestation. Newborn lambs were ventilated targeting a tidal volume of 6-7 mL/kg and a PEEP of 4 cmH(2)O. At 30 min all lambs underwent a PEEP challenge: PEEP was increased by 2 cmH(2)O at 10 min intervals to 10 cmH(2)O then decreased similarly to 4 cmH(2)O. Ventilation parameters, arterial blood flows and pressures were recorded in real-time for 90 min. LPS lambs had higher total protein in bronchoalveolar lavage fluid (p

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