Intramyocardial cell delivery: Observations in murine hearts

Tommaso Poggioli, Padmini Sarathchandra, Nadia Rosenthal, Maria Paola Santini

Research output: Contribution to journalArticleOtherpeer-review

6 Citations (Scopus)

Abstract

Previous studies showed that cell delivery promotes cardiac function amelioration by release of cytokines and factors that increase cardiac tissue revascularization and cell survival. In addition, further observations revealed that specific stem cells, such as cardiac stem cells, mesenchymal stem cells and cardiospheres have the ability to integrate within the surrounding myocardium by differentiating into cardiomyocytes, smooth muscle cells and endothelial cells. Here, we present the materials and methods to reliably deliver noncontractile cells into the left ventricular wall of immunodepleted mice. The salient steps of this microsurgical procedure involve anesthesia and analgesia injection, intratracheal intubation, incision to open the chest and expose the heart and delivery of cells by a sterile 30-gauge needle and a precision microliter syringe. Tissue processing consisting of heart harvesting, embedding, sectioning and histological staining showed that intramyocardial cell injection produced a small damage in the epicardial area, as well as in the ventricular wall. Noncontractile cells were retained into the myocardial wall of immunocompromised mice and were surrounded by a layer of fibrotic tissue, likely to protect from cardiac pressure and mechanical load.

Original languageEnglish
Article numbere51064
JournalJournal of Visualized Experiments
Volume2014
Issue number83
DOIs
Publication statusPublished - 24 Jan 2014

Keywords

  • Cell therapy
  • Fibrotic tissue
  • Grafting
  • Heart
  • Intramyocardial cell injection
  • Issue 83
  • Medicine
  • Stem cells

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