Intragenic dominant suppressors of glp-1, a gene essential for cell- signaling in Caenorhabditis elegans, support a role for cdc10/SW16/ankyrin motifs in GLP-1 function

J. L. Lissemore, P. D. Currie, C. M. Turk, E. M. Maine

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13 Citations (Scopus)


The glp-1 gene product mediates cell-cell interactions required for cell fate specification during development in Caenorhabditis elegans. To identify genes that interact with glp-1, we screened for dominant suppressors of two temperature-sensitive glp-1 alleles and recovered 18 mutations that suppress both germline and embryonic glp-1 phenotypes. These dominant suppressors are tightly linked to glp-1 and do not bypass the requirement for a distal tip cell, which is thought to be the source of a signal that is received and transduced by the GLP-1 protein. Using single-strand conformation polymorphism (SSCP) analysis and DNA sequencing, we found that at least 17 suppressors are second-site intragenic revertants. The suppressors, like the original glp-1(ts) mutations, are all located in the cdc10/SW16/ankyrin domain of GLP-1. cdc10/SW16/ankyrin motifs have been shown to mediate specific protein-protein interactions in other polypeptides. We propose that the glp-1(ts) mutations disrupt contact between GLP-1 and an as yet unidentified target protein(s) and that the dominant suppressor mutations restore appropriate protein-protein interactions.

Original languageEnglish
Pages (from-to)1023-1034
Number of pages12
Issue number4
Publication statusPublished - 1993
Externally publishedYes

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