Intracerebroventricular administration of the β3-adrenoceptor agonist CL 316243 causes Fos immunoreactivity in discrete regions of rat hypothalamus

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Intracerebroventricular (i.c.v.) administration of the β3-AR agonist BRL37344 causes dose dependent decreases in food intake in rats suggesting a role for β3-AR in the central control of feeding. We have conducted experiments investigating the effects of i.c.v. administration of the selective β3-AR agonist CL316243 on Fos expression to determine whether β3-AR stimulation affects neurones within specific brain nuclei. Significantly higher numbers of Fos positive cells were found in the rats treated i.c.v. with CL316243 compared with control rats in the paraventricular hypothalamus, lateral hypothalamic area, ventromedial hypothalamic nucleus and dorsal hypothalamic area. Pre-treatment with the selective β3-AR antagonist SR59230A resulted in a significant decrease in the number of Fos positive cells in all those areas compared with rats treated with CL316243 alone. These experiments demonstrate that i.c.v. administration of selective β3-AR agonist causes neuronal activation in hypothalamic areas important in the central regulation of appetite via a β3-AR mediated effect. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalNeuroscience Letters
Volume290
Issue number3
DOIs
Publication statusPublished - 1 Sep 2000

Keywords

  • Adrenergic
  • Brain, Fos
  • Immunohistochemistry
  • Rat
  • β-Adrenoceptor

Cite this

@article{e9d3e33bc5a74ca0843c8fab46338e0a,
title = "Intracerebroventricular administration of the β3-adrenoceptor agonist CL 316243 causes Fos immunoreactivity in discrete regions of rat hypothalamus",
abstract = "Intracerebroventricular (i.c.v.) administration of the β3-AR agonist BRL37344 causes dose dependent decreases in food intake in rats suggesting a role for β3-AR in the central control of feeding. We have conducted experiments investigating the effects of i.c.v. administration of the selective β3-AR agonist CL316243 on Fos expression to determine whether β3-AR stimulation affects neurones within specific brain nuclei. Significantly higher numbers of Fos positive cells were found in the rats treated i.c.v. with CL316243 compared with control rats in the paraventricular hypothalamus, lateral hypothalamic area, ventromedial hypothalamic nucleus and dorsal hypothalamic area. Pre-treatment with the selective β3-AR antagonist SR59230A resulted in a significant decrease in the number of Fos positive cells in all those areas compared with rats treated with CL316243 alone. These experiments demonstrate that i.c.v. administration of selective β3-AR agonist causes neuronal activation in hypothalamic areas important in the central regulation of appetite via a β3-AR mediated effect. (C) 2000 Elsevier Science Ireland Ltd.",
keywords = "Adrenergic, Brain, Fos, Immunohistochemistry, Rat, β-Adrenoceptor",
author = "Margie Castillo-Mel{\'e}ndez and McKinley, {Michael J.} and Summers, {Roger J.}",
year = "2000",
month = "9",
day = "1",
doi = "10.1016/S0304-3940(00)01359-8",
language = "English",
volume = "290",
pages = "161--164",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier",
number = "3",

}

Intracerebroventricular administration of the β3-adrenoceptor agonist CL 316243 causes Fos immunoreactivity in discrete regions of rat hypothalamus. / Castillo-Meléndez, Margie; McKinley, Michael J.; Summers, Roger J.

In: Neuroscience Letters, Vol. 290, No. 3, 01.09.2000, p. 161-164.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Intracerebroventricular administration of the β3-adrenoceptor agonist CL 316243 causes Fos immunoreactivity in discrete regions of rat hypothalamus

AU - Castillo-Meléndez, Margie

AU - McKinley, Michael J.

AU - Summers, Roger J.

PY - 2000/9/1

Y1 - 2000/9/1

N2 - Intracerebroventricular (i.c.v.) administration of the β3-AR agonist BRL37344 causes dose dependent decreases in food intake in rats suggesting a role for β3-AR in the central control of feeding. We have conducted experiments investigating the effects of i.c.v. administration of the selective β3-AR agonist CL316243 on Fos expression to determine whether β3-AR stimulation affects neurones within specific brain nuclei. Significantly higher numbers of Fos positive cells were found in the rats treated i.c.v. with CL316243 compared with control rats in the paraventricular hypothalamus, lateral hypothalamic area, ventromedial hypothalamic nucleus and dorsal hypothalamic area. Pre-treatment with the selective β3-AR antagonist SR59230A resulted in a significant decrease in the number of Fos positive cells in all those areas compared with rats treated with CL316243 alone. These experiments demonstrate that i.c.v. administration of selective β3-AR agonist causes neuronal activation in hypothalamic areas important in the central regulation of appetite via a β3-AR mediated effect. (C) 2000 Elsevier Science Ireland Ltd.

AB - Intracerebroventricular (i.c.v.) administration of the β3-AR agonist BRL37344 causes dose dependent decreases in food intake in rats suggesting a role for β3-AR in the central control of feeding. We have conducted experiments investigating the effects of i.c.v. administration of the selective β3-AR agonist CL316243 on Fos expression to determine whether β3-AR stimulation affects neurones within specific brain nuclei. Significantly higher numbers of Fos positive cells were found in the rats treated i.c.v. with CL316243 compared with control rats in the paraventricular hypothalamus, lateral hypothalamic area, ventromedial hypothalamic nucleus and dorsal hypothalamic area. Pre-treatment with the selective β3-AR antagonist SR59230A resulted in a significant decrease in the number of Fos positive cells in all those areas compared with rats treated with CL316243 alone. These experiments demonstrate that i.c.v. administration of selective β3-AR agonist causes neuronal activation in hypothalamic areas important in the central regulation of appetite via a β3-AR mediated effect. (C) 2000 Elsevier Science Ireland Ltd.

KW - Adrenergic

KW - Brain, Fos

KW - Immunohistochemistry

KW - Rat

KW - β-Adrenoceptor

UR - http://www.scopus.com/inward/record.url?scp=0343443088&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(00)01359-8

DO - 10.1016/S0304-3940(00)01359-8

M3 - Article

VL - 290

SP - 161

EP - 164

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -