Intracellular production of recombinant serpins in yeast

Dion Kaiserman, Corinne R Hitchen, Vitalina Levina, Stephen P Bottomley, Phillip I Bird

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

1 Citation (Scopus)

Abstract

Yeast are a valuable system for recombinant serpin production due to their ability to synthesize large amounts of heterologous gene products as well as their expression of folding chaperones and lack of endogenous serpin genes. In this chapter, we describe a method for intracellular expression of cytoplasmic serpins in the yeast Pichia pastoris. We also give details on how this system can be exploited to produce polymer-forming mutants of secretory serpins.
Original languageEnglish
Title of host publicationMethods in Enzymology, Volume 501: Serpin Structure and Evolution
EditorsJames C Whisstock, Phillip I Bird
Place of PublicationUSA
PublisherAcademic Press
Pages1 - 12
Number of pages12
ISBN (Print)9780123859501
DOIs
Publication statusPublished - 2011

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