Intracellular production of recombinant serpins in yeast

Dion Kaiserman; Corinne R Hitchen; Vitalina Levina; Stephen P Bottomley; Phillip I Bird

doi:10.1016/B978-0-12-385950-1.00001-8

Intracellular production of recombinant serpins in yeast

Dion Kaiserman, Corinne R Hitchen, Vitalina Levina, Stephen P Bottomley, Phillip I Bird

Biochemistry & Molecular Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

1 Citation (Scopus)

Abstract

Yeast are a valuable system for recombinant serpin production due to their ability to synthesize large amounts of heterologous gene products as well as their expression of folding chaperones and lack of endogenous serpin genes. In this chapter, we describe a method for intracellular expression of cytoplasmic serpins in the yeast Pichia pastoris. We also give details on how this system can be exploited to produce polymer-forming mutants of secretory serpins.

Original language	English
Title of host publication	Methods in Enzymology, Volume 501: Serpin Structure and Evolution
Editors	James C Whisstock, Phillip I Bird
Place of Publication	USA
Publisher	Academic Press
Pages	1 - 12
Number of pages	12
ISBN (Print)	9780123859501
DOIs	https://doi.org/10.1016/B978-0-12-385950-1.00001-8
Publication status	Published - 2011

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10.1016/B978-0-12-385950-1.00001-8

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Intracellular production of recombinant serpins in yeast. / Kaiserman, Dion; Hitchen, Corinne R; Levina, Vitalina et al.
Methods in Enzymology, Volume 501: Serpin Structure and Evolution. ed. / James C Whisstock; Phillip I Bird. USA: Academic Press, 2011. p. 1 - 12.

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Research › peer-review

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T1 - Intracellular production of recombinant serpins in yeast

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AU - Hitchen, Corinne R

AU - Levina, Vitalina

AU - Bottomley, Stephen P

AU - Bird, Phillip I

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AB - Yeast are a valuable system for recombinant serpin production due to their ability to synthesize large amounts of heterologous gene products as well as their expression of folding chaperones and lack of endogenous serpin genes. In this chapter, we describe a method for intracellular expression of cytoplasmic serpins in the yeast Pichia pastoris. We also give details on how this system can be exploited to produce polymer-forming mutants of secretory serpins.

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BT - Methods in Enzymology, Volume 501: Serpin Structure and Evolution

A2 - Whisstock, James C

A2 - Bird, Phillip I

PB - Academic Press

CY - USA

ER -

Intracellular production of recombinant serpins in yeast

Abstract

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