Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

J. L.  Wedding; Hugh Hamlyn Harris; Christie A Bader; Sally E Plush; R. Mak; Massimiliano Massi; Douglas Alexander Brooks; Barry Lai; Stefan Vogt; M. V. Werrett; P V Simpson; Brian W Skelton; Stefano Stagni

doi:10.1039/c6mt00243a

Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

J. L. Wedding, Hugh Hamlyn Harris, Christie A Bader, Sally E Plush, R. Mak, Massimiliano Massi, Douglas Alexander Brooks, Barry Lai, Stefan Vogt, M. V. Werrett, P V Simpson, Brian W Skelton, Stefano Stagni

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)₃(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.

Original language	English
Pages (from-to)	382-390
Number of pages	9
Journal	Metallomics
Volume	9
Issue number	4
DOIs	https://doi.org/10.1039/c6mt00243a
Publication status	Published - 1 Apr 2017
Externally published	Yes

Keywords

Rhenium
Tricarbonyl complexes
Ligands

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10.1039/c6mt00243a

Cite this

Wedding, J. L., Harris, H. H., Bader, C. A., Plush, S. E., Mak, R., Massi, M., Brooks, D. A., Lai, B., Vogt, S., Werrett, M. V., Simpson, P. V., Skelton, B. W., & Stagni, S. (2017). Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging. Metallomics, 9(4), 382-390. https://doi.org/10.1039/c6mt00243a

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title = "Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging",

abstract = "Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.",

keywords = "Rhenium, Tricarbonyl complexes, Ligands",

author = "Wedding, {J. L.} and Harris, {Hugh Hamlyn} and Bader, {Christie A} and Plush, {Sally E} and R. Mak and Massimiliano Massi and Brooks, {Douglas Alexander} and Barry Lai and Stefan Vogt and Werrett, {M. V.} and Simpson, {P V} and Skelton, {Brian W} and Stefano Stagni",

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doi = "10.1039/c6mt00243a",

language = "English",

volume = "9",

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Wedding, JL, Harris, HH, Bader, CA, Plush, SE, Mak, R, Massi, M, Brooks, DA, Lai, B, Vogt, S, Werrett, MV, Simpson, PV, Skelton, BW & Stagni, S 2017, 'Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging', Metallomics, vol. 9, no. 4, pp. 382-390. https://doi.org/10.1039/c6mt00243a

Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging. / Wedding, J. L. ; Harris, Hugh Hamlyn; Bader, Christie A et al.
In: Metallomics, Vol. 9, No. 4, 01.04.2017, p. 382-390.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

AU - Wedding, J. L.

AU - Harris, Hugh Hamlyn

AU - Bader, Christie A

AU - Plush, Sally E

AU - Mak, R.

AU - Massi, Massimiliano

AU - Brooks, Douglas Alexander

AU - Lai, Barry

AU - Vogt, Stefan

AU - Werrett, M. V.

AU - Simpson, P V

AU - Skelton, Brian W

AU - Stagni, Stefano

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.

AB - Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.

KW - Rhenium

KW - Tricarbonyl complexes

KW - Ligands

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DO - 10.1039/c6mt00243a

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AN - SCOPUS:85018776703

SN - 1756-5901

VL - 9

SP - 382

EP - 390

JO - Metallomics

JF - Metallomics

IS - 4

ER -

Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging

Abstract

Keywords

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