Intracellular calcium levels can regulate Importin-dependent nuclear import

Gurpreet Kaur, Jennifer Dung Ly Huynh, David A Jans

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We previously showed that increased intracellular calcium can modulate Importin (Imp)beta1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impalpha/beta1- or Impbeta1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impbeta1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.
Original languageEnglish
Pages (from-to)812 - 817
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume450
Issue number1
DOIs
Publication statusPublished - 2014

Cite this

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abstract = "We previously showed that increased intracellular calcium can modulate Importin (Imp)beta1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impalpha/beta1- or Impbeta1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impbeta1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.",
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Intracellular calcium levels can regulate Importin-dependent nuclear import. / Kaur, Gurpreet; Ly Huynh, Jennifer Dung; Jans, David A.

In: Biochemical and Biophysical Research Communications, Vol. 450, No. 1, 2014, p. 812 - 817.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Kaur, Gurpreet

AU - Ly Huynh, Jennifer Dung

AU - Jans, David A

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N2 - We previously showed that increased intracellular calcium can modulate Importin (Imp)beta1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impalpha/beta1- or Impbeta1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impbeta1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.

AB - We previously showed that increased intracellular calcium can modulate Importin (Imp)beta1-dependent nuclear import of SRY-related chromatin remodeling proteins. Here we extend this work to show for the first time that high intracellular calcium inhibits Impalpha/beta1- or Impbeta1-dependent nuclear protein import generally. The basis of this relates to the mislocalisation of the transport factors Impbeta1 and Ran, which show significantly higher nuclear localization in contrast to various other factors, and RCC1, which shows altered subnuclear localisation. The results here establish for the first time that intracellular calcium modulates conventional nuclear import through direct effects on the nuclear transport machinery.

UR - http://www.sciencedirect.com/science/article/pii/S0006291X14011218

U2 - 10.1016/j.bbrc.2014.06.047

DO - 10.1016/j.bbrc.2014.06.047

M3 - Article

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