Intracellular and in vivo cyanide mapping via surface plasmon spectroscopy of single Au-Ag nanoboxes

Peiyuan Wang, Yujie Bai, Chi Yao, Xiaomin Li, Lei Zhou, Wenxing Wang, Ahmed Mohamed El-Toni, Jian Zi, Dongyuan Zhao, Lei Shi, Fan Zhang

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)


Cyanide is extremely toxic to organisms but difficult to detect in living
biological specimens. Here, we report a new CN− sensing platform based on unmodified Au−Ag alloy nanoboxes that etch in the presence of this analyte, yielding a shift in plasmon frequency that correlates with the analyte concentration. Significantly, when combined with dark field microscopy, these particle probes can be used to measure CN− concentrations in HeLa cells and in vivo in Zebra fish embryos. The limit of detection (LOD) of the novel method is 1 nM (below the acceptable limit defined by the World Health Organization), and finite-difference time-domain (FDTD) calculations are used to understand the CN− induced spectral shifts.
Original languageEnglish
Pages (from-to)2583-2591
Number of pages9
JournalAnalytical Chemistry
Issue number4
Publication statusPublished - 21 Feb 2017
Externally publishedYes

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