Interplay between chromatin remodeling and epigenetic changes during lineage-specific commitment to granzyme B expression

Torsten Juelich, Elissa L Sutcliffe, Alice E Denton, Yiqing He, Peter C Doherty, Christopher R Parish, Steven J. Turner, David Tremethick, Sudha Rao

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

The role of chromatin remodeling and histone posttranslational modifications and how they are integrated to control gene expression during the acquisition of cell-specific functions is poorly understood. We show here that following in vitro activation of CD4+ and CD8+ T lymphocytes, both cell types show rapid histone H3 loss at the granzyme B (gzmB) proximal promoter region. However, despite the gzmB proximal promoter being remodeled in both T cell subsets, only CD8+ T cells express high levels of gzmB and display a distinct pattern of key epigenetic marks, notably differential H3 acetylation and methylation. These data suggest that for high levels of transcription to occur a distinct set of histone modifications needs to be established in addition to histone loss at the proximal promoter of gzmB.

Original languageEnglish
Pages (from-to)7063-7072
Number of pages10
JournalJournal of Immunology
Volume183
Issue number11
DOIs
Publication statusPublished - 1 Dec 2009
Externally publishedYes

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